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Epigenetics and Nuclear Signaling Chromosome Structure Telomeres

Anti-TRF1抗体(ab1423)

  • Datasheet
  • SDS
Reviews (4)Q&A (12)References (40)

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Western blot - Anti-TRF1 antibody (ab1423)
  • Immunocytochemistry - Anti-TRF1 antibody (ab1423)
  • Immunocytochemistry - Anti-TRF1 antibody (ab1423)

Key features and details

  • Rabbit polyclonal to TRF1
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-TRF1抗体
    参阅全部 TRF1 一抗
  • 描述

    兔多克隆抗体to TRF1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, WBmore details
    不适用于: IHC-P
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human TRF1. Human full length recombinant protein expressed in bacteria.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Chromatin

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TRF1 protein (ab116406)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1423于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB (3)
1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
说明
ICC
Use at an assay dependent concentration.
WB
1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • 组织特异性

    Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • 序列相似性

    Contains 1 HTH myb-type DNA-binding domain.
  • 结构域

    The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • 翻译后修饰

    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • 细胞定位

    Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Target information above from: UniProt accession P54274 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7013 Human
    • Omim: 600951 Human
    • SwissProt: P54274 Human
    • Unigene: 442707 Human
    • 别名

      • hTRF1 AS antibody
      • NIMA interacting protein 2 antibody
      • NIMA-interacting protein 2 antibody
      • PIN 2 antibody
      • PIN2 antibody
      • t TRF1 antibody
      • Telomeric protein Pin2 antibody
      • Telomeric protein Pin2/TRF1 antibody
      • Telomeric repeat binding factor (NIMA interacting) 1 antibody
      • Telomeric repeat binding factor 1 antibody
      • Telomeric repeat binding protein 1 antibody
      • Telomeric repeat-binding factor 1 antibody
      • TERF 1 antibody
      • Terf1 antibody
      • TERF1_HUMAN antibody
      • TRBF 1 antibody
      • TRBF1 antibody
      • TRF 1 antibody
      • TRF antibody
      • TTAGGG repeat binding factor 1 antibody
      • TTAGGG repeat-binding factor 1 antibody
      see all

    图片

    • Western blot - Anti-TRF1 antibody (ab1423)
      Western blot - Anti-TRF1 antibody (ab1423)
      Anti-TRF1 antibody (ab1423) at 1/2000 dilution + HL-60 (Human promyelocytic leukemia cell line) whole cell extract

      Observed band size: 60-65 kDa why is the actual band size different from the predicted?



      Western blot labelling TRF1 in HL-60 (Human promyelocytic leukemia cell line) whole cell extract using ab1423 at 1/2000. A single band at 60-65 kD is detected.

    • Immunocytochemistry - Anti-TRF1 antibody (ab1423)
      Immunocytochemistry - Anti-TRF1 antibody (ab1423)

      Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.

    • Immunocytochemistry - Anti-TRF1 antibody (ab1423)
      Immunocytochemistry - Anti-TRF1 antibody (ab1423)
      The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (40)

    发表研究结果有使用 ab1423?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1423 被引用在 40 文献中.

    • Ferreira da Silva J  et al. Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair. Nat Commun 13:760 (2022). IF ; Human . PubMed: 35140211
    • Lu Y  et al. Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis by reducing the polarisation of M1 and M2 macrophages through the activation of EP2 receptors. IET Nanobiotechnol 16:14-24 (2022). PubMed: 34862858
    • Barnes RP  et al. Targeted Formation of 8-Oxoguanine in Telomeres. Methods Mol Biol 2444:141-159 (2022). PubMed: 35290636
    • Ge J  et al. Telomere Dysfunction in Oocytes and Embryos From Obese Mice. Front Cell Dev Biol 9:617225 (2021). IF ; Mouse . PubMed: 33553179
    • Kang S  et al. Transcriptional regulation of telomeric repeat-containing RNA by acridine derivatives. RNA Biol N/A:1-17 (2021). WB ; Human . PubMed: 33749516
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-TRF1 antibody - ChIP Grade

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    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8% resolution gel)
    Sample
    Human Cell lysate - whole cell (hepatocellular carcinoma)
    Specification
    hepatocellular carcinoma
    Treatment
    Inhibition of one gene's expression using lentiviral shRNA
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 16 2013

    Western blot abreview for Anti-TRF1 antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell)
    Loading amount
    100 µg
    Specification
    HeLa cell
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Geun Hyoung Ha

    Verified customer

    提交于 May 27 2009

    Western blot abreview for Anti-TRF1 antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela CELLS)
    Loading amount
    50 µg
    Specification
    Hela CELLS
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Geun-Hyoung Ha

    Verified customer

    提交于 Apr 30 2009

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