Anti-TRF1抗体(ab1423)
Key features and details
- Rabbit polyclonal to TRF1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRF1抗体
参阅全部 TRF1 一抗 -
描述
兔多克隆抗体to TRF1 -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human TRF1. Human full length recombinant protein expressed in bacteria.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB | (3) |
1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
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| 说明 |
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ICC
Use at an assay dependent concentration. |
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WB
1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended. |
靶标
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功能
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. -
组织特异性
Highly expressed and ubiquitous. Isoform Pin2 predominates. -
序列相似性
Contains 1 HTH myb-type DNA-binding domain. -
结构域
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2. -
翻译后修饰
Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. - Information by UniProt
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数据库链接
- Entrez Gene: 7013 Human
- Omim: 600951 Human
- SwissProt: P54274 Human
- Unigene: 442707 Human
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别名
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
see all
图片
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Western blot - Anti-TRF1 antibody (ab1423)Anti-TRF1 antibody (ab1423) at 1/2000 dilution + HL-60 (Human promyelocytic leukemia cell line) whole cell extract
Observed band size: 60-65 kDa why is the actual band size different from the predicted?Western blot labelling TRF1 in HL-60 (Human promyelocytic leukemia cell line) whole cell extract using ab1423 at 1/2000. A single band at 60-65 kD is detected.
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Immunocytochemistry - Anti-TRF1 antibody (ab1423)Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.
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Immunocytochemistry - Anti-TRF1 antibody (ab1423)The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab1423 被引用在 40 文献中.
- Ferreira da Silva J et al. Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair. Nat Commun 13:760 (2022). IF ; Human . PubMed: 35140211
- Lu Y et al. Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis by reducing the polarisation of M1 and M2 macrophages through the activation of EP2 receptors. IET Nanobiotechnol 16:14-24 (2022). PubMed: 34862858
- Barnes RP et al. Targeted Formation of 8-Oxoguanine in Telomeres. Methods Mol Biol 2444:141-159 (2022). PubMed: 35290636
- Ge J et al. Telomere Dysfunction in Oocytes and Embryos From Obese Mice. Front Cell Dev Biol 9:617225 (2021). IF ; Mouse . PubMed: 33553179
- Kang S et al. Transcriptional regulation of telomeric repeat-containing RNA by acridine derivatives. RNA Biol N/A:1-17 (2021). WB ; Human . PubMed: 33749516