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使用敲除细胞株进行验证重组RabMAb

重组Anti-TREM2抗体[EPR26209-22] - BSA and Azide free (ab318263)

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Western blot - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Multiplex immunohistochemistry - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Multiplex immunohistochemistry - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Immunoprecipitation - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
  • Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26209-22] to TREM2 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB, mIHC
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-TREM2抗体[EPR26209-22] - BSA and Azide free
    参阅全部 TREM2 一抗

  • 描述

    兔单克隆抗体[EPR26209-22] to TREM2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IP, WB, mIHCmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Wild-type THP-1 whole cell lysate. IHC-P: Human astrocytoma, Human glioblastoma, Human Alzheimer's cerebrum,HEK-293T transfected with a TREM2 expression vector containing a Myc-His tag, THP-1 cell pellet tissues. MIHC: Human cerebrum and Human astrocytoma tissues. IP: THP-1 cell.

  • 常规说明

    ab318263 is the carrier-free version of ab318262

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318263于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
mIHC
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
mIHC
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.

  • 组织特异性

    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.

  • 疾病相关

    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • TREM 2 antibody
      • TREM-2 antibody
      • TREM2 antibody
      • TREM2_HUMAN antibody
      • TREM2a antibody
      • TREM2b antibody
      • TREM2c antibody
      • Trggering receptor expressed on myeloid cells 2 antibody
      • Trggering receptor expressed on myeloid cells 2a antibody
      • Triggering receptor expressed on monocytes 2 antibody
      • Triggering receptor expressed on myeloid cells 2 antibody
      see all

    图片

    • Western blot - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Western blot - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      All lanes : Anti-TREM2 antibody [EPR26209-22] (ab318262) at 1/1000 dilution

      Lane 1 : Wild-type THP-1 (human monocytic leukemia monocyte) whole cell lysate
      Lane 2 : TREM2 knockout THP-1 whole cell lysate
      Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
      Lane 4 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Performed under reducing conditions.

      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, ab318262 was shown to bind specifically to TREM2.  Target of interest was observed at 30 kDa in wild-type THP-1 cell lysates (lane 1) with no signal observed at this size in TREM2 knockout cell line (lane 2) (lane 2, knockout cell line ab269489 / knockout cell lysate ab269652).

      Negative control: SH-SY5Y, HL-60.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human astrocytoma. The section was incubated with ab318262 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human glioblastoma tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human glioblastoma. The section was incubated with ab318262 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human Alzheimer's cerebrum (PMID: 25186950; : 25186950; PMID: 28592261 ). The section was incubated with ab318262 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a TREM2 expression vector containing a Myc-His tag. (B) HEK-293T transfected with TREM1 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling TREM2 with ab318262 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a TREM2 expression vector containing a Myc-His tag, negative staining on (B) HEK-293T transfected with TREM1 expression vector containing a His tag and (C) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab318262 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) THP-1 (human monocytic leukemia monocyte) cell pellet (B) SH-SY5Y (human neuroblastoma epithelial cell) cell pellet (C) HL-60 (human acute promyelocytic leukemia promyeloblast) cell pellet tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) THP-1 cell pellet, negative staining on (B) SH-SY5Y cell pellet and (C) HL-60 cell pellet. The section was incubated with ab318262 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on human liver (PMID: 12472885). The section was incubated with ab318262 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Multiplex immunohistochemistry - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Multiplex immunohistochemistry - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue staining TREM2 with ab318262 at a 1:100 (5.29 ug/ml) dilution, ab306583 anti-TMEM119 used at 1:2000 (0.255 ug/ml) dilution and ab218309 anti-GFAP used at a 1:1000 (1.325 ug/ml) dilution.

      Panel A: merged staining of anti-TREM2 (green; Opal™ 520), anti-TMEM119 (magenta; Opal™ 690) and anti-GFAP (yellow; Opal™ 570) on human cerebrum.
      Panel B: anti-TREM2 staining microglia in human cerebrum.
      Panel C: anti-TMEM119 staining microglia in human cerebrum.
      Panel D: anti-GFAP staining astrocytes in human cerebrum.
      Nuclear DNA was labeled with DAPI (shown in blue).

      The section was incubated in three rounds of staining: in the order of ab318262, ab306583 and ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

      Nuclear counter stain with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Multiplex immunohistochemistry - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Multiplex immunohistochemistry - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human astrocytoma tissue staining TREM2 with ab318262 at a 1:100 (5.29 ug/ml) dilution, ab306583 anti-TMEM119 used at 1:2000 (0.255 ug/ml) dilution and ab218309 anti-GFAP used at a 1:1000 (1.325 ug/ml) dilution.

      Panel A: merged staining of anti-TREM2 (green; Opal™ 520), anti-TMEM119 (magenta; Opal™ 690) and anti-GFAP (yellow; Opal™ 570) on human astrocytoma.
      Panel B: anti-TREM2 staining microglia in human astrocytoma.
      Panel C: anti-TMEM119 staining microglia in human astrocytoma.
      Panel D: anti-GFAP staining astrocyte in human astrocytoma.
      Nuclear DNA was labeled with DAPI (shown in blue).

      The section was incubated in three rounds of staining: in the order of ab318262, ab306583 and ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

      The immunostaining was performed on a Leica Biosystems BOND™ RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

      Nuclear counter stain with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunoprecipitation - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Immunoprecipitation - Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

      This data was developed using ab318262, the same antibody clone in a different buffer formulation.

      TREM2 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab318262 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318262 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate
      Lane 2: ab318262 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab318262 in THP-1 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 84 seconds..

    • Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)
      Anti-TREM2 antibody [EPR26209-22] - BSA and Azide free (ab318263)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab318263 尚未被引用在任何文献中。

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