重组Anti-TREM2抗体[EPR20243]

• IP

Supplier Data

Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (AB209814)

Immunoprecipitation of TREM2 in THP-1 cells. Lysates were prepared and immunoprecipitation was performed using 2μg of ab209814 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (ab209814) at 2 µg

All lanes:

THP-1 cell lysates

Observed band size: 31 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (AB209814)

TREM2 was immunoprecipitated from 0.35 mg of THP-1 (Human monocytic leukemia cell line) whole cell lysate with ab209814 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209814 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : THP-1 whole cell lysate, 10 μg (Input).

Lane 2 : ab209814 IP in THP-1 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209814 in THP-1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (ab209814)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

Supplier Data

Western blot - Anti-TREM2 antibody [EPR20243] (AB209814)

ab209814 was shown to react with TREM2 in wild-type THP-1 cells in Western blot with loss of signal observed in TREM2 knockout cell line ab269489. 10 μg of concentrated culture media from PMA-treated Wild-type THP-1 and TREM2 knockout cell lysates. Wild-type THP-1 and TREM2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab209814 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-TREM2 antibody [EPR20243] (ab209814) at 1/1000 dilution

Lane 1:

10 μg of concentrated culture media from PMA-treated Wild-type THP-1 lysate at 20 µg

Lane 2:

10 μg of concentrated culture media from PMA-treated TREM2 knock-out THP-1 lysate at 20 µg

Lane 2:

Western blot - Human TREM2 knockout THP-1 cell line (<a href='/products/cell-lines/human-trem2-knockout-thp-1-cell-line-ab269489'>ab269489</a>)

Observed band size: 31 kDa

false

• WB

Lab

Western blot - Anti-TREM2 antibody [EPR20243] (AB209814)

Lanes 1 - 3 : Merged signal (red and green). Green - ab209814 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab209814 was shown to react with TREM2 in wild-type THP-1 cells in Western blot with loss of signal observed in TREM2 knockout cell line ab269489 (TREM2 knockout cell lysate ab269652). Wild-type THP-1 and TREM2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab209814 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-TREM2 antibody [EPR20243] (ab209814) at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human TREM2 knockout THP-1 cell lysate (<a href='/products/cell-lysates/human-trem2-knockout-thp-1-cell-lysate-ab269652'>ab269652</a>) at 20 µg

Lane 3:

HL-60 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-TREM2 antibody [EPR20243] (AB209814)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TREM2 antibody [EPR20243] (ab209814) at 1/1000 dilution

Lane 1:

Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa

false

Exposure time: 4s