Anti-TREM2抗体- C-terminal (ab223684)
Key features and details
- Goat polyclonal to TREM2 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TREM2抗体- C-terminal
参阅全部 TREM2 一抗 -
描述
山羊多克隆抗体to TREM2 - C-terminal -
宿主
Goat -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TREM2 aa 150 to the C-terminus (C terminal) (Cysteine residue). NP_061838.1.
Database link: Q9NZC2 -
阳性对照
- WB: Human cerebellum, frontal cortex and hippocampus lysates. IHC-P: Human lung tissue. ICC/IF: HepG2 cells and U937 cells. Flow cyto: U937 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab223684 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223684于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| Flow Cyt |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
靶标
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功能
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
组织特异性
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
疾病相关
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 54209 Human
- Omim: 605086 Human
- SwissProt: Q9NZC2 Human
- Unigene: 435295 Human
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别名
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody - C-terminal (ab223684)
Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr using ab223684 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody - C-terminal (ab223684)Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using ab223684 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Flow Cytometry - Anti-TREM2 antibody - C-terminal (ab223684)Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr using ab223684 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody - C-terminal (ab223684)Paraffin-embedded human lung tissue stained for TREM2 using ab223684 at 2 µg/ml in immunohistochemical analysis, followed by AP-staining.
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Western blot - Anti-TREM2 antibody - C-terminal (ab223684)All lanes : Anti-TREM2 antibody - C-terminal (ab223684) at 0.3 µg/ml
Lane 1 : Human cerebellum lysate in RIPA buffer
Lane 2 : Human frontal cortex lysate in RIPA buffer
Lane 3 : Human hippocampus lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223684 尚未被引用在任何文献中。