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重组RabMAb

重组Anti-TREM1抗体[EPR22060-229] (ab225861)

Submit a review Submit a question References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
  • Anti-TREM1 antibody [EPR22060-229] (ab225861)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22060-229] to TREM1
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TREM1抗体[EPR22060-229]
    参阅全部 TREM1 一抗

  • 描述

    兔单克隆抗体[EPR22060-229] to TREM1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human colon, stomach and lung tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR22060-229

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225861于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.

  • 组织特异性

    Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻译后修饰

    Glycosylated.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD354 antibody
      • OTTMUSP00000018206 antibody
      • TREM 1 antibody
      • TREM-1 antibody
      • TREM1 antibody
      • TREM1_HUMAN antibody
      • Triggering receptor expressed on monocytes 1 antibody
      • Triggering receptor expressed on myeloid cells 1 antibody
      • Triggering receptor TREM 1 antibody
      • Triggering receptor TREM1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)

      IHC image of TREM1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated overnight at +4°C with ab225861 at 1 µg/ml and ab783, Mouse monoclonal [PG-M1] to CD68, at 5 µg/ml and then detected with an Alexa Fluor® 594 goat anti-rabbit secondary antibody (ab150080) at a 1/1000 dilution (shown in red) and Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)

      IHC image of TREM1 staining in a section of formalin-fixed paraffin-embedded normal human lung*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated overnight at +4°C with ab225861 at 1 µg/ml and ab783, Mouse monoclonal [PG-M1] to CD68, at 5 µg/ml and then detected with an Alexa Fluor® 594 goat anti-rabbit secondary antibody (ab150080) at a 1/1000 dilution (shown in red) and Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TREM1 with ab225861 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on interstitial cells of human colon (PMID: 17853946; PMID: 20933010) is observed. Counterstained with hematoxylin.


      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)

      Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling TREM1 with ab225861 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on interstitial cells and lymphocytes in human stomach (PMID: 17853946; PMID: 20933010) is observed. Counterstained with hematoxylin.


      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (ab225861)

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling TREM1 with ab225861 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on alveolar macrophages in human lung (PMID: 12792857; PMID: 20933010) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-TREM1 antibody [EPR22060-229] (ab225861)
      Anti-TREM1 antibody [EPR22060-229] (ab225861)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (6)

    发表研究结果有使用 ab225861？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225861 被引用在 6 文献中.

    • Ma K  et al. High Expression of Triggering Receptor Expressed on Myeloid Cells 1 Predicts Poor Prognosis in Glioblastoma. Onco Targets Ther 16:331-345 (2023). PubMed: 37274309
    • Griesinger AM  et al. Multi-omic approach identifies hypoxic tumor-associated myeloid cells that drive immunobiology of high-risk pediatric ependymoma. iScience 26:107585 (2023). PubMed: 37694144
    • Sawaya AP  et al. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers. EMBO Rep 23:e54558 (2022). PubMed: 35856334
    • Chu CY  et al. Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy. Theranostics 11:3624-3641 (2021). PubMed: 33664852
    • Brown EE  et al. Mitochondrial oxidative stress in the retinal pigment epithelium (RPE) led to metabolic dysfunction in both the RPE and retinal photoreceptors. Redox Biol 24:101201 (2019). PubMed: 31039480
    • Long J  et al. Development and validation of a TP53-associated immune prognostic model for hepatocellular carcinoma. EBioMedicine 42:363-374 (2019). PubMed: 30885723

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