Anti-TRAP/CD40L抗体[MR-1] (ab99894)

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Flow Cytometry - Anti-TRAP/CD40L antibody [MR-1] (ab99894)

    Key features and details

    • Armenian hamster monoclonal [MR-1] to TRAP/CD40L
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    FITC

    概述

    • 产品名称

      Anti-TRAP/CD40L抗体[MR-1]
      参阅全部 TRAP/CD40L 一抗

    • 描述

      亚美尼亚仓鼠单克隆抗体[MR-1] to TRAP/CD40L

    • 宿主

      Armenian hamster

    • 特异性

      This antibody detects mouse TRAP/CD40L/CD154. a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to Mouse TRAP/CD40L. activated mouse Th1 clone D1.6

    • 阳性对照

      • 6-8 hour activated mouse splenocytes

    • 常规说明


      Functional Application: Blocking T cell costimulation in vivo.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab99894于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use a concentration of 3 µg/ml.
    说明
    Flow Cyt
    Use a concentration of 3 µg/ml.

    靶标

    • 功能

      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

    • 组织特异性

      Specifically expressed on activated CD4+ T-lymphocytes.

    • 疾病相关

      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.

    • 序列相似性

      Belongs to the tumor necrosis factor family.

    • 翻译后修饰

      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.

    • 细胞定位

      Secreted and Cell membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 40L antibody
        • CD154 antibody
        • CD40 antigen ligand antibody
        • CD40 ligand antibody
        • CD40 ligand, soluble form antibody
        • CD40-L antibody
        • CD40L antibody
        • CD40L_HUMAN antibody
        • CD40LG antibody
        • gp39 antibody
        • hCD40L antibody
        • HIGM1 antibody
        • IGM antibody
        • IMD3 antibody
        • T B cell activating molecule antibody
        • T BAM antibody
        • T-cell antigen Gp39 antibody
        • TNF-related activation protein antibody
        • TNFSF5 antibody
        • TrAP antibody
        • Tumor necrosis factor (ligand) superfamily member 5 antibody
        • Tumor necrosis factor ligand superfamily member 5 antibody
        see all

      图片

      • Flow Cytometry - Anti-TRAP/CD40L antibody [MR-1] (ab99894)
        Flow Cytometry - Anti-TRAP/CD40L antibody [MR-1] (ab99894)

        Flow Cytometry analysis of CD154 positive cells (red) from mouse CD154 negative cells (black) using ab99894 at 3 μg/ml.

         

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      客户评价及客户问答

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      Question

      Could you please detail the exact predicted binding sites of the following antibodies: CD40 antibody: ab58612 (Rabbit polyclonal to CD40) - Specifically, where in the C-terminus does this antibody bind - which domain etc? - Can you please issue the sequence of this immunizing peptide? Is it available for purchase? CD40L antibody: ab2391 (Hamster monoclonal to CD40L) - Specifically, where in CD40L does this antibody bind? - Can you please issue the sequence of this immunizing peptide? - Additionally, would you predict that this antibody will recognize mouse CD40L based on the immunizing sequence? CD40L antibody: ab65854 (Rabbit polyclonal to CD40L) - It states that it is raised against a synthetic peptide derived from the N-terminus of human CD40L, but where in the N-terminus does this antibody bind? Can you please issue the sequence of this immunizing peptide? CD40L antibody [MR-1]: ab99894 (Hamster monoclonal to CD40L) - Specifically, which region of CD40L does this antibody interact with? Additionally, can you recommend negative tissue type controls to use for CD40 and CD40L? I would greatly appreciate assistance

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      Answer

      Thank you for contacting Abcam regarding our CD40 and CD40L antibodies. Below please find the requested information: ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that it is a small peptide that surrounds aa246 of human CD40L. Also, I have learned from the laboratory that this antibody will react with mouse and is guaranteed to do so. ab58612 Rabbit Polyclonal to CD40 - The specific sequence is proprietary. Unfortunately, the blocking peptide is not commercially available. ab65854 Rabbit Polyclonal to CD40L - Please contact us for additional immunogen information. ab99894 Hamster monoclonal to CD40L - Unfortunately the epitope has not been mapped for this antibody. Regarding a possible negative control - for CD40 and CD40L I would recommend liver. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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