重组Anti-TRAP/CD40L抗体[EPR20727-307] - BSA and Azide free (ab273509)
![Flow Cytometry - Anti-TRAP/CD40L antibody [EPR20727-307] - BSA and Azide free (ab273509)](https://www.abcam.cn/ps/products/273/ab273509/Images/ab273509-1-anti-trapcd40l-antibody-epr20727307-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Flow Cytometry - Anti-TRAP/CD40L antibody [EPR20727-307] - BSA and Azide free (ab273509)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20727-307] to TRAP/CD40L - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRAP/CD40L抗体[EPR20727-307] - BSA and Azide free
参阅全部 TRAP/CD40L 一抗 -
描述
兔单克隆抗体[EPR20727-307] to TRAP/CD40L - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cytmore details
不适用于: ICC/IF,IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Human peripheral blood mononuclear cells.
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常规说明
ab273509 is the carrier-free version of ab273499.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20727-307 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
组织特异性
Specifically expressed on activated CD4+ T-lymphocytes. -
疾病相关
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 959 Human
- Omim: 300386 Human
- SwissProt: P29965 Human
- Unigene: 592244 Human
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别名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
图片
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Flow Cytometry - Anti-TRAP/CD40L antibody [EPR20727-307] - BSA and Azide free (ab273509)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) treated with 50ng/mL PMA and 500ng/mL Ionomycin for 6h cells labelling TRAP/CD40L with ab273499 at 1/600 dilution (0.1µg) (Right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273499).
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![Anti-TRAP/CD40L antibody [EPR20727-307] - BSA and Azide free (ab273509)](https://www.abcam.cn/ps/products/273/ab273509/Images/ab273509-2-benefits-of-recombinant-antibodies.png)
Anti-TRAP/CD40L antibody [EPR20727-307] - BSA and Azide free (ab273509)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273509 尚未被引用在任何文献中。
