Anti-TRAP/CD40L抗体(ab65854)
Key features and details
- Rabbit polyclonal to TRAP/CD40L
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TRAP/CD40L抗体
参阅全部 TRAP/CD40L 一抗 -
描述
兔多克隆抗体to TRAP/CD40L -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human TRAP/CD40L (N terminal). Different from the relative mouse sequence by two amino acids
Database link: P29965 -
阳性对照
- IHC-P: Rat intestine, human mammary tissues. WB: MCF-7, HeLa, Jurkat, HMY2, COLO320 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65854于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa). |
靶标
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功能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
组织特异性
Specifically expressed on activated CD4+ T-lymphocytes. -
疾病相关
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 959 Human
- Entrez Gene: 21947 Mouse
- Entrez Gene: 84349 Rat
- Omim: 300386 Human
- SwissProt: P29965 Human
- SwissProt: P27548 Mouse
- SwissProt: Q9Z2V2 Rat
- Unigene: 592244 Human
see all -
别名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (ab65854)
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Western blot - Anti-TRAP/CD40L antibody (ab65854)All lanes : Anti-TRAP/CD40L antibody (ab65854) at 0.5 µg/ml
Lane 1 : MCF-7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HMY2 whole cell lysate
Lane 5 : COLO320 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (ab65854)TRAP/CD40L was detected in paraffin-embedded section of human mammary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65854 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab65854 被引用在 15 文献中.
- Abu El-Asrar AM et al. CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy. Int J Mol Sci 24:N/A (2023). PubMed: 37958563
- Zhou A et al. Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils. Front Immunol 14:1171308 (2023). PubMed: 37325657
- Bi H et al. Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway. Cell Death Discov 9:326 (2023). PubMed: 37658042
- Ma J et al. Role of HSP90α in osteoclast formation and osteoporosis development. Exp Ther Med 23:273 (2022). PubMed: 35251339
- Yang B et al. γ-Secretase inhibitors suppress IL-20-mediated osteoclastogenesis via Notch signalling and are affected by Notch2 in vitro. Scand J Immunol 96:e13169 (2022). PubMed: 35384009