Anti-TRAP220/MED1 (phospho T1457)抗体(ab60950)
Key features and details
- Rabbit polyclonal to TRAP220/MED1 (phospho T1457)
- Suitable for: ELISA, ICC/IF, ChIP, IHC-P, WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-TRAP220/MED1 (phospho T1457)抗体
参阅全部 TRAP220/MED1 一抗 -
描述
兔多克隆抗体to TRAP220/MED1 (phospho T1457) -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, ChIP, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human TRAP220/MED1 (phospho T1457). Synthetic phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457 (A-Y-TP-P-Q).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab60950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (2) |
1/500 - 1/1000.
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| ChIP |
Use at an assay dependent concentration. PubMed: 19122196
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| IHC-P |
1/50 - 1/100.
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| WB |
1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/500 - 1/1000. |
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ChIP
Use at an assay dependent concentration. PubMed: 19122196 |
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IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa). |
靶标
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功能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the Mediator complex subunit 1 family. -
翻译后修饰
Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3. - Information by UniProt
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数据库链接
- Entrez Gene: 5469 Human
- Entrez Gene: 19014 Mouse
- Omim: 604311 Human
- SwissProt: Q15648 Human
- SwissProt: Q925J9 Mouse
- Unigene: 643754 Human
- Unigene: 12926 Mouse
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别名
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
see all
图片
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Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950) at 1/500 dilution
Lane 1 : HuvEc cell extracts
treated with Serum (20%, 30mins)
Lane 2 : HuvEc cell extracts
treated with Serum (20%, 30mins) with immunising peptide
Predicted band size: 180 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)ab60950 at 1/50 - 1/100 dilution staining TRAP220/MED1 in human heart by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide -
Immunocytochemistry/ Immunofluorescence - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)ab60950 at 1/500 - 1/1000 dilution staining TRAP220/MED1 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab60950 被引用在 10 文献中.
- Constantin TA et al. The CDK7 inhibitor CT7001 (Samuraciclib) targets proliferation pathways to inhibit advanced prostate cancer. Br J Cancer 128:2326-2337 (2023). PubMed: 37076563
- Ohnuki H et al. Reusable Single Cell for Iterative Epigenomic Analyses. J Vis Exp N/A:N/A (2022). PubMed: 35225292
- Sun X et al. Hippo-YAP signaling controls lineage differentiation of mouse embryonic stem cells through modulating the formation of super-enhancers. Nucleic Acids Res 48:7182-7196 (2020). PubMed: 32510157
- Yamamoto S et al. Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-?B and C/EBPß on stimulation of Toll-like receptor 9. Genes Cells 22:265-276 (2017). PubMed: 28151579
- Waters KM et al. ERK oscillation-dependent gene expression patterns and deregulation by stress response. Chem Res Toxicol 27:1496-503 (2014). WB . PubMed: 25068892
- Gavini CK et al. Leanness and heightened nonresting energy expenditure: role of skeletal muscle activity thermogenesis. Am J Physiol Endocrinol Metab 306:E635-47 (2014). PubMed: 24398400
- Zhao W et al. SIMPL enhancement of tumor necrosis factor-a dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function. PLoS One 8:e61123 (2013). WB, ChIP ; Human . PubMed: 23630580
- Hulf T et al. Epigenetic-induced repression of microRNA-205 is associated with MED1 activation and a poorer prognosis in localized prostate cancer. Oncogene : (2012). PubMed: 22869146
- Persaud SD et al. Gene repressive activity of RIP140 through direct interaction with CDK8. Mol Endocrinol 25:1689-98 (2011). IP ; Mouse . PubMed: 21868449
- Saramäki A et al. Cyclical Chromatin Looping and Transcription Factor Association on the Regulatory Regions of the p21 (CDKN1A) Gene in Response to 1{alpha},25-Dihydroxyvitamin D3. J Biol Chem 284:8073-82 (2009). ChIP ; Human . PubMed: 19122196