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重组

重组Anti-TRAP220/MED1抗体[BLR037F] - BSA free (ab272086)

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Western blot - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
  • Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
  • Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
  • Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR037F] to TRAP220/MED1 - BSA free
  • Suitable for: WB, ICC, IHC-P, IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-TRAP220/MED1抗体[BLR037F] - BSA free
    参阅全部 TRAP220/MED1 一抗

  • 描述

    兔单克隆抗体[BLR037F] to TRAP220/MED1 - BSA free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC, IHC-P, IPmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide within Human TRAP220/MED1 aa 1550-1581 (C terminal). The exact sequence is proprietary. NP_004765.2 and Gene ID 5469.
    Database link: Q15648

  • 阳性对照

    • WB: HEK-293T, MCF7, Hep G2, A549, SW620, SK-MEL-28 and Jurkat whole cell lysates, IP: HeLa whole cell lysate. IHC-P: Human lung carcinoma and Mouse renal carcinoma tissue. ICC: HCT 116 cells.

  • 常规说明

    ab272086 is the BSA-free version of ab243893.

    This product is sold under License from Bethyl Laboratories, Inc.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272086于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • 组织特异性

    Ubiquitously expressed.

  • 序列相似性

    Belongs to the Mediator complex subunit 1 family.

  • 翻译后修饰

    Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Activator-recruited cofactor 205 kDa component antibody
      • ARC205 antibody
      • CRSP1 antibody
      • CRSP200 antibody
      • DRIP205 antibody
      • DRIP230 antibody
      • MED1 antibody
      • MED1_HUMAN antibody
      • Mediator complex subunit 1 antibody
      • Mediator of RNA polymerase II transcription subunit 1 antibody
      • p53 regulatory protein RB18A antibody
      • PBP antibody
      • Peroxisome proliferator-activated receptor-binding protein antibody
      • PPAR binding protein antibody
      • PPAR-binding protein antibody
      • PPARBP antibody
      • PPARGBP antibody
      • RB18A antibody
      • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
      • Thyroid receptor-interacting protein 2 antibody
      • TR-interacting protein 2 antibody
      • Trap220 antibody
      • TRIP-2 antibody
      • TRIP2 antibody
      • Vitamin D receptor-interacting protein complex component DRIP205 antibody
      see all

    图片

    • Western blot - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Western blot - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      All lanes : Anti-TRAP220/MED1 antibody [BLR037F] (ab243893) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 5 : SW620 (Human colon adenocarcinoma cell line) whole cell lysate
      Lane 6 : SK-MEL-28 (Human skin melanoma cell line) whole cell lysate
      Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG

      Exposure time: 3 seconds
    • Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

      TRAP220/MED1 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243893 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243893 at 1/1000 dilution.

      Lane 1: ab243893 IP in HeLa whole cell lysate.

      Lane 2: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Formalin-fixed, paraffin-embedded human small cell lung cancer tissue stained for TRAP220/MED1using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for TRAP220/MED1 using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).

    • Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling TRAF220/MED1 using ab243893 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).

    • Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)
      Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272086？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272086 尚未被引用在任何文献中。

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