AB243893

重组Anti-TRAP220/MED1抗体[BLR037F]

Anti-TRAP220/MED1 antibody [BLR037F]

Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal TRAP220/MED1 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MED1 aa 1550 to C-terminus.

查看别名

ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, MED1, Mediator of RNA polymerase II transcription subunit 1, Activator-recruited cofactor 205 kDa component, Mediator complex subunit 1, Peroxisome proliferator-activated receptor-binding protein, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A, PPAR-binding protein, Trap220, TR-interacting protein 2, TRIP-2

9 Images

Unknown

Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling TRAF220/MED1 using ab243893 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

Flow Cytometry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human MED1 with ab243893 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Formalin-fixed, paraffin-embedded human small cell lung cancer tissue stained for TRAP220/MED1using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Unknown

Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

TRAP220/MED1 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243893 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243893 at 1/1000 dilution. Lane 1 : ab243893 IP in HeLa whole cell lysate.

Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Predicted band size: 168 kDa

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Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Flow cytometry overlay histogram of 4% formaldehyde fixed mIMCD-3 cells permeabilized with 90% methanol labeling human MED1 with ab243893 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for TRAP220/MED1 using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Unknown

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Western blot analysis using ab243893 at 1/1000 dilution.

Lane 1 : HEK-293T whole cell lysate (50 µg).

Lane 2 : MCF7 whole cell lysate (50 µg).

Lane 3 : HepG2 whole cell lysate (50 µg).

Lane 4 : A549 whole cell lysate (50 µg).

Lane 5 : SW 260 whole cell lysate (50 µg).

Lane 6 : SK whole cell lysate (50 µg).

Lane 7 : Jurkat whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Predicted band size: 168 kDa

false

Supplier Data

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

All lanes:

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

HEK293T whole cell lysate at 10 µg

Lane 3:

MCF-7 whole cell lysate at 10 µg

Lane 4:

Hep-G2 whole cell lysate at 10 µg

Lane 5:

A-549 whole cell lysate at 10 µg

Lane 6:

SW620 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 168 kDa

Observed band size: 220 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

All lanes:

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893) at 1/1000 dilution

Lane 1:

EL4 whole cell lysate at 10 µg

Lane 2:

mIMCD-3 whole cell lysate at 10 µg

Lane 3:

RenCa whole cell lysate at 10 µg

Lane 4:

NIH 3T3 whole cell lysate at 10 µg

Lane 5:

TCMK-1 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 167 kDa

Observed band size: 220 kDa

true

Exposure time: 30s

不同偶联物与剂型 (1)

Unconjugated

Anti-TRAP220/MED1 antibody [BLR037F] - BSA free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

BLR037F

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

ICC, IP, Flow Cyt, IHC-P, WB

applications

免疫原

Synthetic Peptide within Human MED1 aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15648

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "" } } }

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产品详情

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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性能和储存信息

形式

Liquid

纯化说明

Recombinant antibody was purified from cell culture supernatant.

存储溶液

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRAP220 also known as MED1 is a protein with a molecular mass of approximately 220 kDa. It plays an important role as a component of the mediator complex which is important for transcription regulation. The expression of TRAP220/MED1 is found in various tissues with higher levels observed in liver lung and heart tissues. This protein acts mechanistically as a bridge between gene-specific transcription factors and RNA polymerase II facilitating the assembly of the pre-initiation complex.

Biological function summary

TRAP220/MED1 functions within the larger mediator complex a multi-protein coactivator essential for gene transcription by RNA polymerase II. It plays an important role in nuclear receptor signaling facilitating the transcriptional activation by various nuclear hormone receptors. MED1 interacts directly with these receptors through its various domains allowing the transcription of target genes responsive to hormonal signals.

Pathways

TRAP220/MED1 contributes significantly to transcriptional activation pathways involving nuclear receptors such as the PPAR (Peroxisome Proliferator-Activated Receptor) and thyroid hormone receptor pathways. It closely interacts with other proteins in these pathways including MED12 and MED14 which form integral parts of the mediator complex's function. Its involvement in these pathways highlights its critical role in lipid metabolism and energy homeostasis.

TRAP220/MED1 is associated with metabolic and proliferative diseases. Its altered expression or mutations can lead to conditions such as obesity and cancer. For instance aberrant MED1 activity or expression levels have connections to breast cancer development through its role in estrogen receptor-mediated gene expression. This correlation emphasizes the protein's potential impact in various pathophysiological contexts driving ongoing research into MED1 as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed : 10406464, PubMed : 11867769, PubMed : 12037571, PubMed : 12218053, PubMed : 12556447, PubMed : 14636573, PubMed : 15340084, PubMed : 15471764, PubMed : 15989967, PubMed : 16574658, PubMed : 9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed : 24245781).

See full target information MED1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409050 PubMed39731339

2024

Super-enhancer Activates Master Transcription Factor NR3C1 Expression and Promotes 5-FU Resistance in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junxian Yu,Mengdi Chen,Qingqing Sang,Fangyuan Li,Zhuoqing Xu,Beiqin Yu,Changyu He,Liping Su,Wentao Dai,Chao Yan,Zheng-Gang Zhu,Jiazeng Xia,Jianfang Li,Haoran Feng,Yunqin Chen,Yuan-Yuan Li,Bingya Liu

Journal of experimental & clinical cancer research : CR 43:270 PubMed39343952

2024

Mediator complex subunit 1 promotes oral squamous cell carcinoma progression by activating MMP9 transcription and suppressing CD8 T cell antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Li,Mengke Sun,Ruimeng Yang,Zheng Wang,Qianyu Zhu,Yue Zhang,Haosun Yang,Zhaosong Meng,Lizhi Hu,Lei Sui

Cell & bioscience 14:54 PubMed38678227

2024

Med1 inhibits ferroptosis and alleviates liver injury in acute liver failure via Nrf2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Ying Lei,Zhi-Hui Li,Deng-Na Lin,Jing Cao,Jun-Feng Chen,Shi-Bo Meng,Jia-Lei Wang,Jing Liu,Jing Zhang,Bing-Liang Lin

EMBO reports 24:e55699 PubMed36629390

2023

The super elongation complex (SEC) mediates phase transition of SPT5 during transcriptional pause release.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghao Guo,Yadi Zhang,Shimin Shuai,Abire Sigbessia,Shaohua Hao,Peng Xie,Xu Jiang,Zhuojuan Luo,Chengqi Lin

Cells 10: PubMed34685704

2021

The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Greisha L Ortiz-Hernandez,Evelyn S Sanchez-Hernandez,Pedro T Ochoa,Catherine C Elix,Hossam R Alkashgari,James R W McMullen,Ubaldo Soto,Shannalee R Martinez,Carlos J Diaz Osterman,Michael Mahler,Sourav Roy,Carlos A Casiano

Frontiers in cellular and infection microbiology 11:693981 PubMed34504806

2021

Transforming Growth Factor-β1 Promotes M1 Alveolar Macrophage Polarization in Acute Lung Injury by Up-Regulating DNMT1 to Mediate the microRNA-124/PELI1/IRF5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqi Wang,Xiaoqing Wang,Hong Zhang,Biao Han,Yuanmei Ye,Mengjie Zhang,Yingbin Wang,Jianjun Xue,Chun'ai Wang

View all publications

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重组Anti-TRAP220/MED1抗体[BLR037F]

Anti-TRAP220/MED1 antibody [BLR037F]

Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Flow Cytometry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Flow Cytometry - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)

Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (AB243893)