AB109323

重组Anti-TRAP1抗体[EPR5381]

Anti-TRAP1 antibody [EPR5381]

RabMAb
Recombinant
了解详情

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(5 Publications)

Rabbit Recombinant Monoclonal TRAP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

查看别名

HSP75, HSPC5, TRAP1, HSP 75, Heat shock protein family C member 5, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (AB109323)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (AB109323)

ab109323 at a 1/100 dilution staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (AB109323)

ab109323 at a 1/100 dilution staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TRAP1 antibody [EPR5381] (AB109323)

All lanes:

Western blot - Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HL60 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 80 kDa

Observed band size: 75 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-TRAP1 antibody [EPR5381] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5381

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRAP1 also known as Tumor Necrosis Factor Receptor-Associated Protein 1 or Heat Shock Protein 75 (Hsp75) is a mitochondrial chaperone protein with a molecular weight of approximately 75 kDa. It functions to protect cells from stress-induced damage by ensuring proper protein folding and preventing aggregation within the mitochondria. TRAP1 is expressed across various tissues with higher levels observed in metabolically active organs such as the heart and liver. Its role extends to regulation of mitochondrial respiration and reactive oxygen species indicating its broad involvement in cellular homeostasis.

Biological function summary

This mitochondrial chaperone plays an important role in maintaining protein homeostasis within the cellular environment. It forms part of a complex with heat shock proteins coordinating cellular defense mechanisms against stress. TRAP1 interacts with other chaperones to stabilize proteins and assist in mitochondrial quality control. Its activities aid in reducing oxidative stress managing apoptosis and regulating mitochondrial dynamics. Through these functions TRAP1 supports cellular survival and metabolic adaptation under conditions of stress.

Pathways

TRAP1 is involved in the regulation of the mitochondrial apoptosis pathway and the oxidative stress response pathway. It interacts with proteins like cytochrome c and inhibitors of apoptosis proteins (IAPs) influencing the release of pro-apoptotic factors and modulation of survival signals. By modulating these pathways TRAP1 affects cellular energy production and stress responses impacting both cellular health and disease states.

TRAP1 shows a direct link to cancer and neurodegenerative diseases. Overexpression of TRAP1 has been associated with various cancers where it promotes cell survival and resistance to apoptosis. In cancer cells TRAP1 interacts with proteins like p53 and Hsp90 contributing to the oncogenic process. In neurodegenerative disorders dysfunction of TRAP1 is linked to increased oxidative stress and mitochondrial dysfunction often relating to proteins such as PINK1 and Parkin which are important in the pathology of Parkinson’s disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.

See full target information TRAP1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1275-1285 PubMed39679449

2024

Tumor necrosis factor receptor-associated protein 1 promotes aerobic glycolysis and cisplatin resistance by regulating the Wnt/β-catenin signaling pathway in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Li,Mengguo Lv,Juan Liu,Qian Sun

International journal of molecular sciences 24: PubMed38139332

2023

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Key,Suzana Gispert,Gabriele Koepf,Julia Steinhoff-Wagner,Marina Reichlmeir,Georg Auburger

Cancer research 79:1369-1382 PubMed30683653

2019

Interplay between TRAP1 and Sirtuin-3 Modulates Mitochondrial Respiration and Oxidative Stress to Maintain Stemness of Glioma Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Kyung Park,Jun-Hee Hong,Young Taek Oh,Sung Soo Kim,Jinlong Yin,An-Jung Lee,Young Chan Chae,Jong Heon Kim,Sung-Hye Park,Chul-Kee Park,Myung-Jin Park,Jong Bae Park,Byoung Heon Kang

Oncology letters 15:5451-5458 PubMed29552185

2018

Overexpression of the mitochondrial chaperone tumor necrosis factor receptor-associated protein 1 is associated with the poor prognosis of patients with colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Gao,Min Li,An-Li Jiang,Rui Sun,Hong-Lin Jin,Hua-Wei Gui,Fei Xiao,Xiang-Wu Ding,Zhen-Ming Fu,Jue-Ping Feng

Molecular & cellular proteomics : MCP 12:2006-20 PubMed23462207

2013

Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study.

Applications

Unspecified application

Species

Unspecified reactive species

Dominik A Megger,Thilo Bracht,Michael Kohl,Maike Ahrens,Wael Naboulsi,Frank Weber,Andreas-Claudius Hoffmann,Christian Stephan,Katja Kuhlmann,Martin Eisenacher,Jörg F Schlaak,Hideo A Baba,Helmut E Meyer,Barbara Sitek

View all publications

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重组Anti-TRAP1抗体[EPR5381]