重组Anti-TRAP alpha/TRAPA抗体[EPR5603] (ab133238)
![Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)](https://www.abcam.cn/ps/products/133/ab133238/Images/ab133238-146642-anti-trap-alpha-antibody-epr5603-western-blot.jpg "Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5603] to TRAP alpha/TRAPA
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRAP alpha/TRAPA抗体[EPR5603]
参阅全部 TRAP alpha/TRAPA 一抗 -
描述
兔单克隆抗体[EPR5603] to TRAP alpha/TRAPA -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human TRAP alpha/TRAPA aa 100-200.
Database link: P43307 -
阳性对照
- A431, HL60, and T47D lysates; HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR5603 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133238于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa). |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
-
功能
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins. -
序列相似性
Belongs to the TRAP-alpha family. -
结构域
Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6745 Human
- Entrez Gene: 107513 Mouse
- Entrez Gene: 361233 Rat
- Omim: 600868 Human
- SwissProt: P43307 Human
- SwissProt: Q9CY50 Mouse
- SwissProt: Q7TPJ0 Rat
- Unigene: 114033 Human
see all -
别名
- DKFZp781N23103 antibody
- FLJ14232 antibody
- FLJ22100 antibody
see all
图片
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Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)All lanes : Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : T47D cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)](https://www.abcam.cn/ps/products/133/ab133238/Images/ab133238-146641-anti-trap-alpha-antibody-epr5603-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)Intracellular flow cytometric analysis of TRAP alpha/TRAPA in permeabilized HepG2 cells, using ab133238 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)](https://www.abcam.cn/ps/products/133/ab133238/Images/ab133238-3-benefits-of-recombinant-antibodies.png)
Anti-TRAP alpha/TRAPA antibody [EPR5603] (ab133238)
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab133238 被引用在 13 文献中.
- Zhang H et al. Hepcidin-induced reduction in iron content and PGC-1ß expression negatively regulates osteoclast differentiation to play a protective role in postmenopausal osteoporosis. Aging (Albany NY) 13:11296-11314 (2021). PubMed: 33820875
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862
- Zhu H et al. Overexpression of PIK3R1 Promotes Bone Formation by Regulating Osteoblast Differentiation and Osteoclast Formation. Comput Math Methods Med 2021:2909454 (2021). PubMed: 34691235
- Ge YW et al. The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo. Oxid Med Cell Longev 2020:1404915 (2020). PubMed: 32587656
- Zhu J et al. Instability and excessive mechanical loading mediate subchondral bone changes to induce osteoarthritis. Ann Transl Med 8:350 (2020). PubMed: 32355794