Anti-Translin/TSN抗体(ab71775)
Key features and details
- Rabbit polyclonal to Translin/TSN
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Translin/TSN抗体
参阅全部 Translin/TSN 一抗 -
描述
兔多克隆抗体to Translin/TSN -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Translin/TSN.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71775于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 26 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
1/500 - 1/1000. Predicted molecular weight: 26 kDa. |
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IHC-P
Use at an assay dependent concentration. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 7247 Human
- Entrez Gene: 22099 Mouse
- Entrez Gene: 60381 Rat
- Omim: 600575 Human
- SwissProt: Q15631 Human
- SwissProt: Q62348 Mouse
- Unigene: 75066 Human
- Unigene: 426637 Mouse
see all -
形式
Cellular Localisation: Cytoplasm. Nucleus -
别名
- BCLF 1 antibody
- BCLF1 antibody
- Component 3 of promoter of RISC antibody
see all
图片
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Western blot - Anti-Translin/TSN antibody (ab71775)All lanes : Anti-Translin/TSN antibody (ab71775) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Translin/TSN antibody (ab71775)Immunohistochemical staining of paraffin-embedded human brain tissue using Translin/TSN antibody (ab71775) at 1/100 dilution. Staining performed in absence (left) and presence (right) of blocking peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Translin/TSN antibody (ab71775)ICC/IF image of ab71775 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71775, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab71775 被引用在 1 文献中.
- Hu J et al. Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice. Biol Reprod 98:102-114 (2018). PubMed: 29161344