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Anti-Transglutaminase 2抗体(ab421)

Reviews (1)Q&A (5)References (27)
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)

    Key features and details

    • Rabbit polyclonal to Transglutaminase 2
    • Suitable for: WB, IHC-P, ELISA, IHC-Fr, ICC/IF
    • Reacts with: Mouse, Rat, Guinea pig, Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Transglutaminase 2抗体
      参阅全部 Transglutaminase 2 一抗

    • 描述

      兔多克隆抗体to Transglutaminase 2

    • 宿主

      Rabbit

    • 特异性

      This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).

    • 经测试应用

      适用于: WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details

    • 种属反应性

      与反应: Mouse, Rat, Guinea pig, Human

    • 免疫原

      Full length native protein (purified) (Guinea Pig).

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab421于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    1/300 (See Abreview).
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 21359202
    ICC/IF
    1/1000.
    说明
    WB
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    1/300 (See Abreview).
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 21359202
    ICC/IF
    1/1000.

    靶标

    • 功能

      Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

    • 序列相似性

      Belongs to the transglutaminase superfamily. Transglutaminase family.
    • Information by UniProt

    • 数据库链接

      • 别名

        • ALPHA SUBUNIT antibody
        • C polypeptide antibody
        • EC 2.3.2.13 antibody
        • epididymis secretory protein Li 45 antibody
        • G alpha h antibody
        • G[a]h antibody
        • Gh CLASS G ALPHA h antibody
        • GNAH antibody
        • GNAH G PROTEIN antibody
        • H POLYPEPTIDE antibody
        • HEL-S-45 antibody
        • Protein glutamine gamma glutamyltransferase 2 antibody
        • Protein-glutamine gamma-glutamyltransferase 2 antibody
        • TG 2 antibody
        • TG(C) antibody
        • TG2 antibody
        • TGase C antibody
        • TGase H antibody
        • TGase-2 antibody
        • TgaseII antibody
        • TGC antibody
        • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
        • TGM2 antibody
        • TGM2_HUMAN antibody
        • Tissue transglutaminase antibody
        • Transglutaminase 2 antibody
        • Transglutaminase 2 C polypeptide antibody
        • Transglutaminase C antibody
        • Transglutaminase H antibody
        • Transglutaminase-2 antibody
        • tTG antibody
        • tTGas antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)
        Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)
        ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      文献 (27)

      发表研究结果有使用 ab421？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab421 被引用在 27 文献中.

      • Orr AN  et al. Transglutaminases Are Active in Perivascular Adipose Tissue. Int J Mol Sci 22:N/A (2021). PubMed: 33808023
      • Sheftel CM & Hernandez LL Serotonin stimulated parathyroid hormone related protein induction in the mammary epithelia by transglutaminase-dependent serotonylation. PLoS One 15:e0241192 (2020). PubMed: 33095824
      • Yang P  et al. TGM2 interference regulates the angiogenesis and apoptosis of colorectal cancer via Wnt/ß-catenin pathway. Cell Cycle 18:1122-1134 (2019). PubMed: 31010374
      • Yu W  et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
      • Dib A  et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Transglutaminase 2 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (lung)
      Specification
      lung
      Blocking step
      Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Miss. OLIVIA FROMIGUE

      Verified customer

      提交于 Aug 08 2005

      Question

      All of the data links for the human protein refer to KERATINOCYTE or EPIDERMAL transglutaminase (TGM1) yet your relevance section discusses TISSUE transglutaminase. Of the proteins with transglutaminase activity, (see Exp Mol Med 31: 5-19. 1999 for instance) what was the immunogen for this Ab? We are interested in detecting via western blot either TGase 1 OR TGase 3.

      Read More
      Answer

      Thank you for your enquiry and your patience in awaiting a response. This product is raised against tissue TGM (TGM2). I have updated the data sheet to reflect this and added data base links relating to the correct target. If you have nay further questions then please do not hesitate to get back in touch with me.

      Read More

      Question

      When this antibody was tested in Western blotting, what type of lysate(s) were used? Has this antibody ever been tested with human squamous epithelial samples?

      Read More
      Answer

      This antibody was tested in WB with lysates from guinea pig liver. It has never specifically been tested with human squamous epithelial samples. Ab421 does cross-react with Human and it is recommended for use at a dilution of 1/200 - 1/5000.

      Read More

      Question

      Customer would like to know: Has this anitbody been tested fro cross-reactivity with other isoforms?

      Read More
      Answer

      Ab421 was raised using guinea pig tissue Tgase (full length native protein (purified)). This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-3). If you have any more questions please contact us again.

      Read More

      Question

      Could I know if Transglutaminase antibody (ab421) is useful on frozen section?

      Read More
      Answer

      This antibody has been tested and characterized on formalin-fixed paraffin-embedded liver tissue. We do not have data on frozen sections, however it is likely that this product will work on acetone or ethanol-fixed tissue section as well.

      Read More

      Question

      Is this tissue transglutaminase or keratinocyte TGTASE I?

      Read More
      Answer

      This antibody recognises cellular/tissue transglutaminase. The immunogen used was purified transglutaminase from guinea pig liver. 'TG1' referred to this being the first transglutaminase antibody to be added to our catalog, not the isoform of the antigen - it is ambiguous, sorry for the confusion and we have now amended the antibody name.

      Read More

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