Anti-Transferrin Receptor (phospho S24)抗体(ab61021)
Key features and details
- Rabbit polyclonal to Transferrin Receptor (phospho S24)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Transferrin Receptor (phospho S24)抗体
参阅全部 Transferrin Receptor 一抗 -
描述
兔多克隆抗体to Transferrin Receptor (phospho S24) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human Transferrin Receptor (phospho S24).
Database link: P02786 -
阳性对照
- Human brain tissue 293 cells, treated with PMA
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61021于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
疾病相关
Immunodeficiency 46 -
序列相似性
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
翻译后修饰
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
细胞定位
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- Unigene: 529618 Human
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别名
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor (phospho S24) antibody (ab61021)Immunohistochemical analysis of paraffin-embedded human brain tissue using ab61021 at a dilution of 1/50.
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Western blot - Anti-Transferrin Receptor (phospho S24) antibody (ab61021)All lanes : Anti-Transferrin Receptor (phospho S24) antibody (ab61021)
Lane 1 : extracts from 293 cells, treated
with PMA (125ng/ml, 30mins)
Lane 2 : extracts from 293 cells, treated
with PMA (125ng/ml, 30mins) with Immunizing phospho-peptide
Predicted band size: 85 kDa
Observed band size: 85 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab61021 被引用在 1 文献中.
- Lee J et al. Investigation of Transcriptional Gene Profiling in Normal Murine Hair Follicular Substructures Using Next-Generation Sequencing to Provide Potential Insights Into Skin Disease. Cell Transplant 25:377-99 (2016). PubMed: 25995029