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Cardiovascular Blood Serum Proteins

Anti-Transferrin Receptor抗体[MEM-189] (ab1086)

  • Datasheet
  • SDS
Reviews (4)Q&A (9)References (28)

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Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (ab1086)

    Key features and details

    • Mouse monoclonal [MEM-189] to Transferrin Receptor
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    概述

    • 产品名称

      Anti-Transferrin Receptor抗体[MEM-189]
      参阅全部 Transferrin Receptor 一抗
    • 描述

      小鼠单克隆抗体[MEM-189] to Transferrin Receptor
    • 宿主

      Mouse
    • 特异性

      The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
    • 经测试应用

      适用于: Flow Cytmore details
      不适用于: ICC/IF
    • 种属反应性

      与反应: Human
    • 免疫原

      Tissue, cells or virus corresponding to Human Transferrin Receptor. KG1 human acute myelogenous leukaemia cell line

    • 阳性对照

      • Flow Cyt: K562 cells.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 8.0
      Preservative: 0.097% Sodium azide
      Constituent: Tris buffer
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      MEM-189
    • 同种型

      IgG1
    • 研究领域

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Stem Cells
      • Neural Stem Cells
      • Surface Molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Erythrocytic Lineage
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1086于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt (1)
    Use a concentration of 2 µg/ml.
    说明
    Flow Cyt
    Use a concentration of 2 µg/ml.
    应用说明
    Is unsuitable for ICC/IF.

    靶标

    • 功能

      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • 疾病相关

      Immunodeficiency 46
    • 序列相似性

      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • 翻译后修饰

      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • 细胞定位

      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P02786 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7037 Human
      • Omim: 190010 Human
      • SwissProt: P02786 Human
      • Unigene: 529618 Human
      • 别名

        • CD 71 antibody
        • CD71 antibody
        • CD71 antigen antibody
        • IMD46 antibody
        • OTTHUMP00000208523 antibody
        • OTTHUMP00000208524 antibody
        • OTTHUMP00000208525 antibody
        • p90 antibody
        • sTfR antibody
        • T9 antibody
        • TFR 1 antibody
        • TfR antibody
        • TfR1 antibody
        • TFR1_HUMAN antibody
        • TFRC antibody
        • TR antibody
        • Transferrin receptor (p90 CD71) antibody
        • Transferrin receptor protein 1, serum form antibody
        • Trfr antibody
        see all

      图片

      • Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (ab1086)
        Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (ab1086)

        Flow Cytometry analysis of K562 cells labelling Transferrin with ab1086 at 2 μg/ml.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (28)

      发表研究结果有使用 ab1086?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1086 被引用在 28 文献中.

      • Shan X  et al. The Protective Effect of Cyanidin-3-Glucoside on Myocardial Ischemia-Reperfusion Injury through Ferroptosis. Oxid Med Cell Longev 2021:8880141 (2021). PubMed: 33628391
      • Zeng X  et al. Benefits of Iron Chelators in the Treatment of Parkinson's Disease. Neurochem Res 46:1239-1251 (2021). PubMed: 33646533
      • Zhang Y  et al. Salvia miltiorrhiza (SM) Injection Ameliorates Iron Overload-Associated Cardiac Dysfunction by Regulating the Expression of DMT1, TfR1, and FP1 in Rats. Evid Based Complement Alternat Med 2021:6864723 (2021). PubMed: 34135983
      • Song Z  et al. Ruscogenin induces ferroptosis in pancreatic cancer cells. Oncol Rep 43:516-524 (2020). PubMed: 31894321
      • Di Marco A  et al. Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System. Cells 9:N/A (2020). PubMed: 32316221
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 13 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Transferrin Receptor antibody [MEM-189]

      Inconclusive
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      African green monkey Cell (Kidney)
      Permeabilization
      Yes - 0.2% triton x 100
      Specification
      Kidney
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jul 27 2017

      Flow Cytometry abreview for Anti-Transferrin Receptor antibody [MEM-189]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Fixation
      None
      Permeabilization
      No
      Sample
      Human Cell (PC-3 Prostrate cancer cell line)
      Specification
      PC-3 Prostrate cancer cell line
      Gating Strategy
      Cells gated for live and singlets
      Preparation
      Cell harvesting/tissue preparation method: Cells detached with PBS-based cell dissociation buffer, collected by centrifugation and re-suspended in PBS + 2% FBS
      Sample buffer: PBS + 2% FBS
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Nov 05 2013

      Question

      .
      I'm using Anti-Transferrin Receptor antibody [MEM-189] (ab1086) for Western blot. I tested it against mouse brain tissue inreducing conditions (I used beta mercaptoethanol as denaturing agent); run on aSDS-PAGE in SDS running buffer; transferon a nitrocellulose membrane. Blockingwith 5% milk solution; using ab1086 at 1:800 over night in blocking buffer. Washing in TBST and incubatingwith anti rabbit as secondary antibody for 1 h.
      I have not gotten any band.I would like to know if i can use the antibody also in reducing conditions (the productcould recognize reducedmonomer at 85 kDa?) and would liketo have some informations aboutblocking,primary antibody concentration andreference bands.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 11 2012

      Answer

      Thank you for contacting us.

      According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual protocol as we do not have any other restrictions on the datasheet. As a side note, we usually use 3%BSA as a blocking agent as we find that it gives a significant stronger signal with monoclonal antibodies.

      According to SwissProt, this protein has a molecular weight of 85 kDa when unmodified. However, as it has many glycosylation sides, I would expect it to run higher; the glycosylation status will influence the weight.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Sep 11 2012

      Question

      I would like to know what site the antibody-antigen (sample) binds? Is it the C-terminal or N-terminal? Or another site of the antigen?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 10 2012

      Answer

      The epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

      Read More

      Abcam Scientific Support

      回复于 Sep 10 2012

      Question

      I am a interested in purchasing a primary anti-transferrin receptor antibody (ab1086) which, according to the datasheet, reacts with human, mouse and rat. However, because this antibody is raised in mouse I am worried it will react to more than my protein of interest when using mouse samples. This is an issue that I ran into in the past and I had to introduce an additional blocking step to eliminate non-specific binding of such primary antibody with mouse proteins. Is this something that I should take into account with this antibody as well? and if so what kind of blocking do I have to do?
      Many thanks in advance
      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 14 2012

      Answer

      Thank you for contacting us.

      Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

      https://www.abcam.com/index.html?pageconfig=resource&rid=11465

      We have kit which is perfect for mouse on mouse staining; please have a look at the datasheet

      https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 May 14 2012

      Question

      Would these products
      (ab1086, ab84036, ab9179, ab8598) detect Trf1 and Trf2?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 12 2012

      Answer

      Thank you for your enquiry.

      We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivity whatsoever.

      I hope this information will help settle any concerns that you may have.

      Read More

      Abcam Scientific Support

      回复于 Apr 12 2012

      Question

      Thank you for your help. I have stored them as the product information indicated, long term in the -20. But I understand keeping the freeze thaws down. I am running some more westerns soon and will include TFRC ab1086 in the bunch. I will keep you posted on the results. If the bands come out at the wrong molecular weight I will be able to get a refund, but not actually return the antibodies? Also, out of curiosity, why is this particular antibody to be used on non reduced samples? Thanks again,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 29 2012

      Answer

      Thank you for bringing that to my attention.
      I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab1086 used under non-reducing conditions should recognize the homodimer at greater than 170kDa after post translation modifications. Did you see any bands in that range?
      If used in reducing conditions it seems that the product should pick up the band around 85kDa (before PTM). The datasheet is not very clear about this. When repeating your experiments I would recommend looking for the larger weight. It may also be useful to try reducing conditions as well.
      I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

      Read More

      Abcam Scientific Support

      回复于 Feb 29 2012

      Question

      Great to hear I can return them. They have been stored in the -20 since arrival. I am not sure what information you need about the original vial so I will give you what I have and see if you have more questions. I lysed the cells in SDS free RIPA buffer with protease inhibitor tablets EDTA free from Roche. Centrifuge to remove DNA. BCA for protein concentration. 10ug protein with 4x loading dye invitrogen, no beta mercaptoethanol. Run on a nupage gel in MOPS SDS running buffer. Transfer to a nitrocellulose blot. Block in Licor brand blocking buffer, use ab1086 at 1:800 over night in blocking buffer and beta actin at 1:5000. Wash in TBST. Incubate in licor anti mouse and rabbit 1 hr. wash in TBST, read on scanner. I will look into getting the order number for the original order, but that may take a little time. I will email when I have that information. I do know that it arrived on if that could narrow the search for you. Let me know if there is any other information you need.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 28 2012

      Answer

      Thank you for your response.

      I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience that this might cause.

      May I suggest however that you repeat your experiment using one of these vials? I have had the opportunity to review your protocol, which looks fine. If your results are the same (a band at less than 1/2 the expected weight) then we might conclude that the issue is with the product. In which case I might refund or replace all the products without need for return.

      Please let me know if this is acceptable for you and if you have any questions.

      Read More

      Abcam Scientific Support

      回复于 Feb 28 2012

      Question

      I recently started using the Abcam antibody AB1086 for TFRC.  I ordered one tube and used it and got a positive signal on a western blot using the non reducing conditions indicated on the data sheet provided.  I recently ordered more to use in a FACS experiment as a control.  I have now realized that the band I was taking as positive signal is not of the proper size.  TFRC is 84kD and the band I see is at about 45. I would like to return the three new tubes I bought if possible as this antibody is not suitable for my experiment. . I am attaching a PPT slide with the western image. Please let me know if you need any more information and how you would like to precede. material from any computer.  Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 27 2012

      Answer

      I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimately a problem with one of our product I am waiving the standard 20% restocking fee for you.

      Before I may process this return however I will need a little information:

      Your order was for three vials of ab1086. Have any of these been opened?

      How have these products been stored since delivery?

      I would also like to discuss the original vial of ab1086 with you and perhaps issue a free of charge replacement or refund for that product. In order to do this I will need some information about your experiments with this product. Could you please send me the protocol used?

      Would you also be able to send me the order number for the original vial?

      Please let me know if you have any questions.

      Read More

      Abcam Scientific Support

      回复于 Feb 27 2012

      Question

      Dear,

      We recently bought the antibody (ab1086 ) from your company.

      We tested it against a 67NR mouse cell line ( mammary carcinoma ).

      We had already seen with another CD71 directly FITC labeled antibody that this cell line was positive for CD71.

      But if we use your antibody in the amount you advise ( 2 µg/ml ) or in a higher concentration we cannot find a positive signal.

      As a secondary antibody we use Goat anti mouse-apc which works in the positive crtl.

      In the data sheet it says that there is cross reactivity with mouse.

      Can you help us with this problem?

      With kind regards,

      Birgit Senden







      Birgit Senden
      Research Technician
      Internal Medicine / Haematology
      mailto:b.senden@maastrichtuniversity.nl
      http://www.maastrichtuniversity.nl

      P. Debeyelaan 25, 6229 HX Maastricht
      P.O. Box 616, 6200 MD Maastricht, The Netherlands
      T +31 43 3881740 F +31 43 3884164


      Note: the email addresses have changed!


      Please consider your environmental responsibility before printing this e-mail.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 26 2012

      Answer

      Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from ab1086.

      I would like to reassure you that In the event that a product is not functioning in the applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

      I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

      I would appreciate if you could also provide an image which would help us to assess the results.

      Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


      Order Details
      Antibody code:

      Lot number:

      Purchase order number
      or preferably Abcam order number:

      General Information
      Antibody storage conditions (temperature/reconstitution etc)

      Description of the problem (high background, low signal, non-specific satining etc.)
      Sample (Species/Tissue/Cell Type/Cell Line etc.)


      Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


      Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


      Permeabilization step


      Blocking conditions (Buffer/time period, Blocking agent etc.)


      Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


      Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


      Detection method


      Positive and negative controls used (please specify)


      Optimization attempts (problem solving)
      How many times have you tried the IHC?


      Have you run a "No Primary" control?
      Yes No

      Do you obtain the same results every time?
      Yes No


      What steps have you altered?


      Additional Notes


      We would appreciate if you are also able to provide and image which woudl help us to assess the results

      Read More

      Abcam Scientific Support

      回复于 Jan 26 2012

      1-10 of 13 Abreviews or Q&A

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