重组Anti-Transferrin Receptor抗体[EPR4013] (ab109259)

Anti-Transferrin Receptor antibody [EPR4013] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109259 was shown to bind specifically to Transferrin Receptor. A band was observed at 90 kDa in wild-type K562 cell lysates with no signal observed at this size in Tfrc knockout cell line. To generate this image, wild-type and Tfrc knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.