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Anti-Transferrin抗体[HTF-14] (ab769)

Reviews (1)Q&A (3)References (10)
Western blot - Anti-Transferrin antibody [HTF-14] (ab769)
  • Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [HTF-14] (ab769)

Key features and details

  • Mouse monoclonal [HTF-14] to Transferrin
  • Suitable for: ELISA, RIA, ICC, WB, Functional Studies, Sandwich ELISA, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Transferrin抗体[HTF-14]
    参阅全部 Transferrin 一抗

  • 描述

    小鼠单克隆抗体[HTF-14] to Transferrin

  • 宿主

    Mouse

  • 特异性

    This antibody recognizes an epitope located in the N-terminal domain of human transferrin.

  • 经测试应用

    适用于: ELISA, RIA, ICC, WB, Functional Studies, Sandwich ELISA, IP, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rabbit, Pig不与反应: Sheep, Horse, Cow, Dog, Carp

  • 免疫原

    Full length native protein (purified) (Pig).

  • 阳性对照

    • WB: Human transferrin. IHC-P: Human Placenta

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab769于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.

Works best under non reducing conditions.
Functional Studies
Use at an assay dependent concentration.

Effectively blocks the transferrin-transferrin receptor interactions.
Sandwich ELISA
Use a concentration of 1 µg/ml.

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.
IP
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml.
说明
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Works best under non reducing conditions.
Functional Studies
Use at an assay dependent concentration.

Effectively blocks the transferrin-transferrin receptor interactions.
Sandwich ELISA
Use a concentration of 1 µg/ml.

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.
IP
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

  • 组织特异性

    Expressed by the liver and secreted in plasma.

  • 疾病相关

    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.

  • 序列相似性

    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Transferrin antibody [HTF-14] (ab769)
      Western blot - Anti-Transferrin antibody [HTF-14] (ab769)
      All lanes : Anti-Transferrin antibody [HTF-14] (ab769)

      Lane 1 : Human Transferrin – 8 µl (reducing conditions) at 5 µg
      Lane 2 : HumanTransferrin – 5 µl (reducing conditions) at 3 µg
      Lane 3 : Human Transferrin – 3 µl (reducing conditions) at 1 µg
      Lane 4 : Human Transferrin – 3 µl (non reducing conditions) at 1 µg
      Lane 5 : Human Transferrin – 5 µl (non reducing conditions) at 3 µg
      Lane 6 : Human Transferrin - 8 µl (non reducing conditions) at 5 µg


      The sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.

      Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer

      SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl

      Marker – 3 µl

      WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes

      Detection:

      Blocking solution: 1xPBS/5% BSA, 4°C, overnight

      Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)

      Washing solution: 1x PBS/0.1% Tween

      Secondary Ab: IRDye® 800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.

      Detection: Li-COR odyssey

      No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.

    • Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)
      Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)

      Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [HTF-14] (ab769)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [HTF-14] (ab769)

      Immunohistochemistry staining of human placenta (paraffin sections) using ab769 10 μg/ml

    文献 (10)

    发表研究结果有使用 ab769？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab769 被引用在 10 文献中.

    • Zhang LJ  et al. Age-Related Loss of Innate Immune Antimicrobial Function of Dermal Fat Is Mediated by Transforming Growth Factor Beta. Immunity 50:121-136.e5 (2019). PubMed: 30594464
    • Lu R  et al. Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7. EBioMedicine 36:29-38 (2018). PubMed: 30243490
    • Griveau A  et al. A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment. Cancer Cell 33:874-889.e7 (2018). PubMed: 29681511
    • Lo Giudice MC  et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . PubMed: 27845346
    • Shi R  et al. Identification and functional study of osteosarcoma metastasis marker genes. Oncol Lett 10:1848-1852 (2015). WB ; Human . PubMed: 26622763
    • Salvati A  et al. Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface. Nat Nanotechnol 8:137-43 (2013). PubMed: 23334168
    • Pitek AS  et al. Transferrin coated nanoparticles: study of the bionano interface in human plasma. PLoS One 7:e40685 (2012). Dot blot . PubMed: 22829881
    • Williams M  et al. Mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition without migration of a cell population. Dev Dyn 241:270-83 (2012). PubMed: 22170865
    • Bartek J  et al. Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. Br J Haematol 59:435-41 (1985). PubMed: 2578807
    • Bartek J  et al. Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity. Immunol Lett 4:231-5 (1982). PubMed: 7095835

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Transferrin antibody [HTF-14]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - other (artery biopsy)
    Loading amount
    20 µg
    Specification
    artery biopsy
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 06 2007

    Question

    We wish to create a lateral flow assay, I am shopping for antibodies against human transferrin. Please help me on following questions.  1) Antibody pairing information to generate lateral flow test for detecting human transferrin  2) If such pairing information is available, we would like to know the performance of such pair in lateral flow test, i.e. the sensitivity and specificity   (crossreactivity with transferrin from other species).  3) Positive control information to validate the antibody performance in lateral flow test.

    Read More
    Answer

    Lateral flow is not a tested application, however it is very much like sandwich ELISA so any ab pair used in sELISA should work in LF. We do offer a matched antibody for use with ab769 this is ab9538. Gold conjugation is generally used in LF. We do not carry gold conjugated anti-Transferrin, so the customer will need to conjugate one ab for use as the colored marker. 1) Ab9538 is tested and guaranteed in sELISA. For use in LT we recommend using this ab9583 as the test line and ab769 as colored particle. We do not have a gold conjugation kit however these may be found at online. Control line will be Mouse IgG1 monoclonal antibody [NCM1] - Isotype control (ab27479). You will need a detection system which will be able to read the gold particles, a flatbed scanner will likely work. 2) We do not test in LT. Ab769 is known to Reacts with: Rabbit, Human, Pig and not with Sheep, Horse, Cow, Dog or Carp. AB9538 is known to react with human and Marmoset; other species have not been tested. 3) Transferrin protein (Active) ab83560 has been tested with and is the positive control for sELISA with this pair.

    Read More

    Question

    Customer kindly contacted us regarding the molecular weight of ab116604, ab129194, ab769.

    Read More
    Answer

    Thank you for contacting Abcam.

    Since all three antibodies have IgG (or a IgG subclass) isotypes, the molecular weight of each is approximately 160kDa (23-25kDa for both light chains, and 53kDa for both heavy chains).

    Read More

    Question

    Here are the additional information for which you asked : 1) Is your plasma sample from human (please specify species)? *Yes* 2) How long has ab769 been stored at 4C? The storage instructions are: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles 4) *2 weeks* 3) How did you block your membrane - please specify blocking agent (and %) and blocking time? *Milk 10%* Could you also provide the purchase order number for this product = *192677* You will also find below the history of our prehundred e-mails where all this information was dejà mentioned ! Bests regards

    Read More
    Answer

    Thank you for the requested information. I have re-examined our record and I was not able to find this information in our past correspondences with you. In regards to the purchase order number "192677", this was an order for ab7355 (Acinus antibody) placed by Patricia from the Inserm. Could you please check this information again and provide the correct PO# for ab769? Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement if the product has been purchased within 90 days. Again, I apologize for any inconveniences and I look forward to your reply.

    Read More

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