Anti-Transcription factor AP-2-alpha抗体(ab52222)
Key features and details
- Rabbit polyclonal to Transcription factor AP-2-alpha
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Transcription factor AP-2-alpha抗体
参阅全部 Transcription factor AP-2-alpha 一抗 -
描述
兔多克隆抗体to Transcription factor AP-2-alpha -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken
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免疫原
Synthetic peptide corresponding to Human Transcription factor AP-2-alpha.
Database link: P05549 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. -
疾病相关
Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies. -
序列相似性
Belongs to the AP-2 family. -
结构域
The WW-binding motif mediates interaction with WWOX. -
翻译后修饰
Sumoylated on Lys-10; which inhibits transcriptional activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 395982 Chicken
- Entrez Gene: 7020 Human
- Entrez Gene: 21418 Mouse
- Entrez Gene: 306862 Rat
- Omim: 107580 Human
- SwissProt: P05549 Human
- SwissProt: P34056 Mouse
- SwissProt: P58197 Rat
see all -
别名
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer-binding protein 2-alpha antibody
- Activator protein 2 antibody
see all
图片
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Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222)All lanes : Anti-Transcription factor AP-2-alpha antibody (ab52222) at 1/500 dilution
Lane 1 : COLO 205 (Human colon adenocarcinoma cell line) extracts
Lane 2 : COLO 205 (Human colon adenocarcinoma cell line) extracts with immunizing peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (ab52222)Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab52222.
Left: - peptide. Right: + peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab52222 被引用在 27 文献中.
- Ruff KLM et al. Allele-specific transcription factor binding in a cellular model of orofacial clefting. Sci Rep 12:1807 (2022). PubMed: 35110662
- Xu H et al. Silencing of lncRNA DLEU1 inhibits tumorigenesis of ovarian cancer via regulating miR-429/TFAP2A axis. Mol Cell Biochem 476:1051-1061 (2021). PubMed: 33170430
- Fan X et al. TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. Elife 10:N/A (2021). PubMed: 33554859
- Xiong Y et al. TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-ß signaling pathway. Cell Death Dis 12:352 (2021). PubMed: 33824285
- Ramamoorthy K et al. Effect of chronic alcohol exposure on gut vitamin B7 uptake: involvement of epigenetic mechanisms and effect of alcohol metabolites. Am J Physiol Gastrointest Liver Physiol 321:G123-G133 (2021). PubMed: 34077272