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Anti-Transcription factor AP-2-alpha抗体(ab52222)

Reviews (8)Q&A (3)References (29)
Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (ab52222)

Key features and details

  • Rabbit polyclonal to Transcription factor AP-2-alpha
  • Suitable for: ELISA, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] - BSA and Azide free (ab236043)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Transcription factor AP-2-alpha抗体
    参阅全部 Transcription factor AP-2-alpha 一抗

  • 描述

    兔多克隆抗体to Transcription factor AP-2-alpha

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken

  • 免疫原

    Synthetic peptide corresponding to Human Transcription factor AP-2-alpha.
    Database link: P05549

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52222于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
WB (2)
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P (2)
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.

  • 疾病相关

    Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies.

  • 序列相似性

    Belongs to the AP-2 family.

  • 结构域

    The WW-binding motif mediates interaction with WWOX.

  • 翻译后修饰

    Sumoylated on Lys-10; which inhibits transcriptional activity.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Activating enhancer binding protein 2 alpha antibody
    • Activating enhancer-binding protein 2-alpha antibody
    • Activator protein 2 antibody
    • AP 2 transcription factor antibody
    • AP 2alpha antibody
    • AP-2 antibody
    • AP-2 transcription factor antibody
    • AP2 antibody
    • AP2 Transcription Factor antibody
    • AP2-alpha antibody
    • AP2A_HUMAN antibody
    • AP2TF antibody
    • BOFS antibody
    • FLJ51761 antibody
    • TFAP 2 antibody
    • TFAP 2A antibody
    • TFAP2 antibody
    • TFAP2A antibody
    • Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody
    • Transcription factor AP-2-alpha antibody
    • Transcription factor AP2 alpha antibody
    see all

图片

  • Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222)
    Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222)
    All lanes : Anti-Transcription factor AP-2-alpha antibody (ab52222) at 1/500 dilution

    Lane 1 : COLO 205 (Human colon adenocarcinoma cell line) extracts
    Lane 2 : COLO 205 (Human colon adenocarcinoma cell line) extracts with immunizing peptide

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (ab52222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (ab52222)

    Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab52222.

    Left: - peptide. Right: + peptide.

文献 (29)

发表研究结果有使用 ab52222？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52222 被引用在 29 文献中.

  • Karttunen K  et al. Transposable elements as tissue-specific enhancers in cancers of endodermal lineage. Nat Commun 14:5313 (2023). PubMed: 37658059
  • Ruff KLM  et al. Allele-specific transcription factor binding in a cellular model of orofacial clefting. Sci Rep 12:1807 (2022). PubMed: 35110662
  • Thomas R  et al. Glycan Epitope and Integrin Expression Dynamics Characterize Neural Crest Epithelial-to-Mesenchymal Transition (EMT) in Human Pluripotent Stem Cell Differentiation. Stem Cell Rev Rep 18:2952-2965 (2022). PubMed: 35727432
  • Xu H  et al. Silencing of lncRNA DLEU1 inhibits tumorigenesis of ovarian cancer via regulating miR-429/TFAP2A axis. Mol Cell Biochem 476:1051-1061 (2021). PubMed: 33170430
  • Fan X  et al. TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. Elife 10:N/A (2021). PubMed: 33554859
View all Publications for this product

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Transcription factor AP-2-alpha antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Embryonic stem cells)
Permeabilization
Yes - methanol
Specification
Embryonic stem cells
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 21 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Transcription factor AP-2-alpha antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryo skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Embryo skin
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Sep 21 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Transcription factor AP-2-alpha antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Embryonic spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Embryonic spinal cord
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Sep 21 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Transcription factor AP-2-alpha antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human cervical cell lines)
Permeabilization
Yes - triton
Specification
human cervical cell lines
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 14 2011

ChIP abreview for Anti-Transcription factor AP-2-alpha antibody

 Good
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (Cervical cancer cell lines)
Negative control
Human primary keratinocytes
Specification
Cervical cancer cell lines
Detection step
Semiquantitative PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
Tumor cell lines Hela
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 03 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Transcription factor AP-2-alpha antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human keratinocytes)
Permeabilization
Yes - 0.1% triton
Specification
human keratinocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 20 2010

Western blot abreview for Anti-Transcription factor AP-2-alpha antibody

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human keratinocytes)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
Human keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 16 2010

Question

Anti-AP2 alpha antibody - ChIP Grade (ab52222) Can you tell me what Immunogen was used to generate this antibody. We wish to use it with species other than human and want to know its conservation as well as its chance of cross-reacting with other similar AP-2 familly members Thanks

Read More
Answer

Thank you for contacting us.

The exact immunogen sequence is not available to us as the originating laboratory considers it proprietary, but we at least know that it is within the final 30 amino acids of the C terminus of the human protein, Uniprot accession P05549 (http://www.uniprot.org/uniprot/P05549).

AMDKMYLSNNPNSHTDNNAKSSDKEEKHRK.

This may be sufficient for your alignments. If not, please send the sequences or accession numbers of the proteins you need to align, and I will forward them to the originating lab for alignment to obtain the percentage identities.

Read More

Question

Hi There,
I have an anti body ab52222 that I wish to use for a chip assay however could you please advise me to as which kit I should purchase from your company that works well with this antibody.
Thanks for your time
Kind Regards

Read More
Answer

Thank you for your inquiry.

I am happy to confirm that ab500 (ChIP Kit) is suitable for this antibody since it can be used with rabbit antibodies.

https://www.abcam.com/ChIP-Kit-ab500.html (or use the following: https://www.abcam.com/ChIP-Kit-ab500.html).

This kit has not been experimentally tested with ab52222, but since ab52222 is tested and guaranteed for CHIP, the combination of this kit and ab52222 is also guaranteed.

I hope this information is helpful and wish you good luck for your experiments.

Read More

Question

I would like to know if your Anti-AP2 alpha antibody - ChIP Grade (ab52222) might detect chicken AP-2 alpha. Is there sufficient conservation between the peptide and the chicken AP2-2 alpha (NP_990425.1)? Thanks

Read More
Answer

Thank you for contacting us.

Reactivity with chicken AP2-2 alpha is predicted based on 100% conservation of the immunogen in the chicken protein NP_990425.1.

However, we do not have data confirming this, and reactivity with chicken AP2-2 alpha is not guaranteed.

Please do not hesitate to contact us if you need any more advice or information.

Read More

1-10 of 11 Abreviews or Q&A

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