JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB52222

Anti-Transcription factor AP-2-alpha抗体

Anti-Transcription factor AP-2-alpha antibody

4

(8 Reviews)

|

(29 Publications)

Rabbit Polyclonal Transcription factor AP-2-alpha antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human TFAP2A.

查看别名

AP2TF, TFAP2, TFAP2A, Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (AB52222)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (AB52222)

Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab52222.

Left : - peptide. Right : + peptide.

Western blot - Anti-Transcription factor AP-2-alpha antibody (AB52222)
  • WB

Unknown

Western blot - Anti-Transcription factor AP-2-alpha antibody (AB52222)

All lanes:

Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222) at 1/500 dilution

Lane 1:

COLO 205 (Human colon adenocarcinoma cell line) extracts

Lane 2:

COLO 205 (Human colon adenocarcinoma cell line) extracts with immunizing peptide

Predicted band size: 48 kDa,76 kDa

Observed band size: 48 kDa,80 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ELISA, IHC-P

applications

免疫原

Synthetic Peptide within Human TFAP2A. The exact immunogen used to generate this antibody is proprietary information.

P05549

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

您是否考虑以下替代产品？

AB236043

Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] - BSA and Azide free

0
0 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Transcription factor AP-2-alpha also known as TFAP2A or AP-2 is a protein acting as an important regulator in cellular processes. It weighs approximately 52 kDa. TFAP2A is known for its role in binding specific DNA sequences to regulate target gene transcription. It expresses highly in neural crest-derived tissues as well as in the placenta breast and skin. This transcription factor plays an essential role in embryonic development cell proliferation and differentiation.
Biological function summary

TFAP2A contributes significantly to the regulation of gene expression involved in essential developmental and cellular processes. It acts as part of a complex with other transcription factors allowing it to coordinate the expression of a variety of genes linked to cell growth and apoptosis. AP-2 also regulates genes involved in neural development and skin morphogenesis. Its interaction with other factors such as AP-2γ and AP-2β enhances its functionality indicating a complex mechanism of action.

Pathways

TFAP2A engages in critical signalling circuits such as the MAPK and Wnt pathways which are essential for cell cycle regulation and embryonic development. Within these pathways related proteins like AP-2γ and AP-594 interact with TFAP2A further illustrating its role in signal transduction. These relationships highlight the importance of TFAP2A in orchestrating cellular responses to growth signals and environmental cues.

TFAP2A has links to a range of pathological conditions including cancer and neural crest-related disorders. In melanoma for example TFAP2A's regulatory role affects genes associated with tumor growth and metastasis. The protein's interaction with other transcription factors like AP-647 may influence cancer progression and therapy response. Additionally the dysregulation of TFAP2A affects neural crest development potentially leading to congenital disorders. Understanding these interactions assists in unraveling the molecular basis of such diseases and informs therapeutic strategies.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
See full target information TFAP2A
style
left-column

文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5313 PubMed37658059

2023

Transposable elements as tissue-specific enhancers in cancers of endodermal lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Konsta Karttunen,Divyesh Patel,Jihan Xia,Liangru Fei,Kimmo Palin,Lauri Aaltonen,Biswajyoti Sahu

Stem cell reviews and reports 18:2952-2965 PubMed35727432

2022

Glycan Epitope and Integrin Expression Dynamics Characterize Neural Crest Epithelial-to-Mesenchymal Transition (EMT) in Human Pluripotent Stem Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ria Thomas,Vishal Menon,Rakesh Mani,Jan Pruszak

Scientific reports 12:1807 PubMed35110662

2022

Allele-specific transcription factor binding in a cellular model of orofacial clefting.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina L M Ruff,Ronja Hollstein,Julia Fazaal,Frederic Thieme,Jan Gehlen,Elisabeth Mangold,Michael Knapp,Julia Welzenbach,Kerstin U Ludwig

Frontiers in cardiovascular medicine 8:678401 PubMed34239903

2021

Elastogenesis Correlates With Pigment Production in Murine Aortic Valve Leaflets.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D Hutcheson,Florian Schlotter,Michael D Creager,Xiaoshuang Li,Tan Pham,Payal Vyas,Hideyuki Higashi,Simon C Body,Masanori Aikawa,Sasha A Singh,Lidia Kos,Elena Aikawa

American journal of physiology. Gastrointestinal and liver physiology 321:G123-G133 PubMed34077272

2021

Effect of chronic alcohol exposure on gut vitamin B7 uptake: involvement of epigenetic mechanisms and effect of alcohol metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Kalidas Ramamoorthy,Subrata Sabui,Padmanabhan Srinivasan,Saleh Al-Juburi,Quang Pham,Brian D Chu,Rita D Simoes,James M Fleckenstein,Hamid M Said

Cell death & disease 12:352 PubMed33824285

2021

TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlu Xiong,Yangbo Feng,Jinbo Zhao,Jie Lei,Tianyun Qiao,Yongsheng Zhou,Qiang Lu,Tao Jiang,Lintao Jia,Yong Han

eLife 10: PubMed33554859

2021

TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Fan,V Pragathi Masamsetti,Jane Qj Sun,Kasper Engholm-Keller,Pierre Osteil,Joshua Studdert,Mark E Graham,Nicolas Fossat,Patrick Pl Tam

Molecular and cellular biochemistry 476:1051-1061 PubMed33170430

2020

Silencing of lncRNA DLEU1 inhibits tumorigenesis of ovarian cancer via regulating miR-429/TFAP2A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huiying Xu,Lingyan Wang,Xiuli Jiang

Investigative ophthalmology & visual science 61:3 PubMed33006594

2020

Long Noncoding RNA OIP5-AS1 Promotes Cell Apoptosis and Cataract Formation by Blocking POLG Expression Under Oxidative Stress

Applications

Unspecified application

Species

Unspecified reactive species

Ruihua Jing,Bo Ma,Tiantian Qi,Conghui Hu,Chongbing Liao,Chan Wen,Yongping Shao,Cheng Pei

Acta pharmacologica Sinica 41:1357-1365 PubMed32415222

2020

Chromatin accessibility analysis reveals that TFAP2A promotes angiogenesis in acquired resistance to anlotinib in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Le-le Zhang,Jun Lu,Rui-Qi Liu,Min-Juan Hu,Yi-Ming Zhao,Sheng Tan,Shu-Yuan Wang,Bo Zhang,Wei Nie,Yu Dong,Hua Zhong,Wei Zhang,Xiao-Dong Zhao,Bao-Hui Han
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB108311

Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)]

primary-antibodies

transcription-factor-ap-2-alpha-antibody-epr26882-ab108311

5

(2 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com