AB52222

Anti-Transcription factor AP-2-alpha抗体

Name: Transcription factor AP-2-alpha抗体 (ab52222)| Abcam中文官网
Brand: Abcam Limited
SKU: AB52222
Price: 5076 CNY
Availability: InStock
Rating: 4 (8 reviews)

Anti-Transcription factor AP-2-alpha antibody

(8 Reviews)

(36 Publications)

Rabbit Polyclonal Transcription factor AP-2-alpha antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human TFAP2A.

查看别名

AP2TF, TFAP2, TFAP2A, Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (AB52222)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (AB52222)

Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab52222.

Left : - peptide. Right : + peptide.

Unknown

Western blot - Anti-Transcription factor AP-2-alpha antibody (AB52222)

All lanes:

Western blot - Anti-Transcription factor AP-2-alpha antibody (ab52222) at 1/500 dilution

Lane 1:

COLO 205 (Human colon adenocarcinoma cell line) extracts

Lane 2:

COLO 205 (Human colon adenocarcinoma cell line) extracts with immunizing peptide

Predicted band size: 48 kDa,76 kDa

Observed band size: 48 kDa,80 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P, ELISA

applications

免疫原

Synthetic Peptide within Human TFAP2A. The exact immunogen used to generate this antibody is proprietary information.

P05549

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Transcription factor AP-2-alpha also known as TFAP2A or AP-2 is a protein acting as an important regulator in cellular processes. It weighs approximately 52 kDa. TFAP2A is known for its role in binding specific DNA sequences to regulate target gene transcription. It expresses highly in neural crest-derived tissues as well as in the placenta breast and skin. This transcription factor plays an essential role in embryonic development cell proliferation and differentiation.

Biological function summary

TFAP2A contributes significantly to the regulation of gene expression involved in essential developmental and cellular processes. It acts as part of a complex with other transcription factors allowing it to coordinate the expression of a variety of genes linked to cell growth and apoptosis. AP-2 also regulates genes involved in neural development and skin morphogenesis. Its interaction with other factors such as AP-2γ and AP-2β enhances its functionality indicating a complex mechanism of action.

Pathways

TFAP2A engages in critical signalling circuits such as the MAPK and Wnt pathways which are essential for cell cycle regulation and embryonic development. Within these pathways related proteins like AP-2γ and AP-594 interact with TFAP2A further illustrating its role in signal transduction. These relationships highlight the importance of TFAP2A in orchestrating cellular responses to growth signals and environmental cues.

TFAP2A has links to a range of pathological conditions including cancer and neural crest-related disorders. In melanoma for example TFAP2A's regulatory role affects genes associated with tumor growth and metastasis. The protein's interaction with other transcription factors like AP-647 may influence cancer progression and therapy response. Additionally the dysregulation of TFAP2A affects neural crest development potentially leading to congenital disorders. Understanding these interactions assists in unraveling the molecular basis of such diseases and informs therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.

See full target information TFAP2A

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

JID innovations : skin science from molecules to population health 5:100394 PubMed40786655

2025

Identification of Keratin 5-Expressing Fibroblasts for Regenerating Keratinocytes in the Necrotic Skin Graft.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshikazu Hirose,Asaka Miura,Yuki Kobayashi,Takashi Shimbo,Yuya Ouchi,Tomomi Kitayama,Tomoya Mori,Akio Tanaka,Manabu Fujimoto,Kotaro Saga,Katsuto Tamai

eNeuro 12: PubMed40701803

2025

Is Required for Peripheral Nerve Function and the Injury Response.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Belfiore,Anjali Balakrishnan,Yohannes Soenjaya,Hussein Ghazale,Alexandra Moffat,Dawn Zinyk,Lakshmy Vasan,Yacine Touahri,Yutaka Amemiya,Tak-Ho Chu,Morgan G Stykel,Arun Seth,Satoshi Okawa,Christine E M Demore,Rajiv Midha,Jeff Biernaskie,Carol Schuurmans

eLife 13: PubMed39641974

2024

Lineage-specific intersection of endothelin and GDNF signaling in enteric nervous system development.

Applications

Unspecified application

Species

Unspecified reactive species

Denise M Poltavski,Alexander T Cunha,Jaime Tan,Henry M Sucov,Takako Makita

iScience 27:109875 PubMed38774716

2024

RAD21 deficiency drives corneal to scleral differentiation fate switching via upregulating WNT9B.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Liu,Benxiang Qi,Guanghui Liu,Haoyun Duan,Zongyi Li,Zhaoying Shi,Yonglong Chen,Wai Kit Chu,Qingjun Zhou,Bi Ning Zhang

Stem cell research & therapy 15:4 PubMed38167128

2024

Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luo,Kaiwen Wu,Xiaolong Zhang,Hongyang Wang,Qiuju Wang

Nature communications 14:5313 PubMed37658059

2023

Transposable elements as tissue-specific enhancers in cancers of endodermal lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Konsta Karttunen,Divyesh Patel,Jihan Xia,Liangru Fei,Kimmo Palin,Lauri Aaltonen,Biswajyoti Sahu

Stem cell reviews and reports 18:2952-2965 PubMed35727432

2022

Glycan Epitope and Integrin Expression Dynamics Characterize Neural Crest Epithelial-to-Mesenchymal Transition (EMT) in Human Pluripotent Stem Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ria Thomas,Vishal Menon,Rakesh Mani,Jan Pruszak

Scientific reports 12:1807 PubMed35110662

2022

Allele-specific transcription factor binding in a cellular model of orofacial clefting.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina L M Ruff,Ronja Hollstein,Julia Fazaal,Frederic Thieme,Jan Gehlen,Elisabeth Mangold,Michael Knapp,Julia Welzenbach,Kerstin U Ludwig

Viruses 13: PubMed34452398

2021

Inhibitors of Venezuelan Equine Encephalitis Virus Identified Based on Host Interaction Partners of Viral Non-Structural Protein 3.

Applications

Unspecified application

Species

Unspecified reactive species

Allison Bakovic,Nishank Bhalla,Farhang Alem,Catherine Campbell,Weidong Zhou,Aarthi Narayanan

Frontiers in cardiovascular medicine 8:678401 PubMed34239903

2021

Elastogenesis Correlates With Pigment Production in Murine Aortic Valve Leaflets.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D Hutcheson,Florian Schlotter,Michael D Creager,Xiaoshuang Li,Tan Pham,Payal Vyas,Hideyuki Higashi,Simon C Body,Masanori Aikawa,Sasha A Singh,Lidia Kos,Elena Aikawa

View all publications

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Anti-Transcription factor AP-2-alpha抗体

Anti-Transcription factor AP-2-alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody (AB52222)

Western blot - Anti-Transcription factor AP-2-alpha antibody (AB52222)