重组Anti-TRAF6BP/TAX1BP1抗体[EPR13287(B)] - C-terminal (ab176572)
![Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)](https://www.abcam.cn/ps/products/176/ab176572/Images/ab176572-515326-anti-traf6bptax1bp1-antibody-epr13287-b--c-terminal-western-blot-wild-type-hek-293-cell-human.jpg "Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13287(B)] to TRAF6BP/TAX1BP1 - C-terminal
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRAF6BP/TAX1BP1抗体[EPR13287(B)] - C-terminal
参阅全部 TRAF6BP/TAX1BP1 一抗 -
描述
兔单克隆抗体[EPR13287(B)] to TRAF6BP/TAX1BP1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human TRAF6BP/TAX1BP1 aa 750 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary. Isoform 1
Database link: Q86VP1 -
阳性对照
- HepG2, A549, 293T and U937 cell lysates; A549 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13287(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - BSA and Azide free (ab238935)
- PE Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab317908)
- APC Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal (ab317947)
- Alexa Fluor® 488 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal (ab317988)
- Alexa Fluor® 594 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal (ab318030)
- Alexa Fluor® 555 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal (ab318072)
- Alexa Fluor® 647 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal (ab318113)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176572于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 91 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/100. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 91 kDa. |
靶标
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功能
Inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. Degraded by caspase-3-like family proteins upon TNF-induced apoptosis. May also play a role in the pro-inflammatory cytokine IL-1 signaling cascade. -
组织特异性
Expressed in all tissues tested. - Information by UniProt
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数据库链接
- Entrez Gene: 8887 Human
- Entrez Gene: 52440 Mouse
- Entrez Gene: 246244 Rat
- Omim: 605326 Human
- SwissProt: Q86VP1 Human
- SwissProt: Q3UKC1 Mouse
- SwissProt: Q66HA4 Rat
- Unigene: 34576 Human
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别名
- 1200003J11Rik antibody
- 1700069J21Rik antibody
- Aa1076 antibody
see all
图片
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Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)All lanes : Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572) at 1/2000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TAX1BP1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Anti-TAX1BP1 antibody [EPR13287(B)] (ab176572) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176572 was shown to bind specifically to TAX1BP1. A band was observed at 95 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAX1BP1 knockout cell line. To generate this image, wild-type and TAX1BP1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)](https://www.abcam.cn/ps/products/176/ab176572/Images/ab176572-204300-anti-traf6bp-antibody-epr13287b-c-terminal-western-blot.jpg)
Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)All lanes : Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)](https://www.abcam.cn/ps/products/176/ab176572/Images/ab176572-204298-anti-traf6bp-antibody-epr13287b-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)Immunofluorescence analysis of A549 cells labeling TRAF6BP/TAX1BP1 using ab176572 at a 1/50 dilution (green). DAPI nuclear staining (blue).
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![Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)](https://www.abcam.cn/ps/products/176/ab176572/Images/ab176572-3-benefits-of-recombinant-antibodies.png)
Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab176572 被引用在 8 文献中.
- Zhang TM et al. TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression. Autophagy 19:805-821 (2023). PubMed: 35920704
- Kuno S & Iwai K Oxygen modulates iron homeostasis by switching iron sensing of NCOA4. J Biol Chem 299:104701 (2023). PubMed: 37059186
- Taraborrelli L et al. Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer. Nat Commun 14:5945 (2023). PubMed: 37741832
- Huyghe J et al. ATG9A prevents TNF cytotoxicity by an unconventional lysosomal targeting pathway. Science 378:1201-1207 (2022). PubMed: 36520901
- Kuno S et al. Iron-induced NCOA4 condensation regulates ferritin fate and iron homeostasis. EMBO Rep 23:e54278 (2022). PubMed: 35318808
- Liu ZZ et al. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. Autophagy 17:2766-2782 (2021). PubMed: 33143524
- Lim J et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. Elife 8:N/A (2019). PubMed: 31287416
- Choi YB et al. TAX1BP1 Restrains Virus-Induced Apoptosis by Facilitating Itch-Mediated Degradation of the Mitochondrial Adaptor MAVS. Mol Cell Biol 37:N/A (2017). PubMed: 27736772