AB176572

重组Anti-TRAF6BP/TAX1BP1抗体[EPR13287(B)] - C-terminal

Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal

RabMAb
Recombinant
KO Validated
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(11 Publications)

Knockout Tested Rabbit Recombinant Monoclonal TRAF6BP/TAX1BP1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.

查看别名

T6BP, PRO0105, TAX1BP1, Tax1-binding protein 1, TRAF6-binding protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (AB176572)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (AB176572)

Immunofluorescence analysis of A549 cells labeling TRAF6BP/TAX1BP1 using ab176572 at a 1/50 dilution (green). DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (AB176572)

All lanes:

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

U937 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 91 kDa

true

Lab

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (AB176572)

Anti-TAX1BP1 antibody [EPR13287(B)] (ab176572) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176572 was shown to bind specifically to TAX1BP1. A band was observed at 95 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAX1BP1 knockout cell line. To generate this image, wild-type and TAX1BP1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (ab176572) at 1/2000 dilution

Lanes 1 - 2:

Western blot at 20 µg

Lane 2:

Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (<a href='/products/cell-lysates/human-tax1bp1-traf6bp-knockout-hek-293t-cell-lysate-ab257726'>ab257726</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 91 kDa

Observed band size: 95 kDa

false

不同偶联物与剂型 (7)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
578 PE

PE Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
660 APC

APC Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
Carrier free

Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13287(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRAF6BP also known as TAX1BP1 is a cytoplasmic protein that participates in important signaling pathways. It has an approximately 75 kDa molecular weight and mainly expresses in immune-related cells including macrophages and lymphocytes. TRAF6BP works as an adaptor protein binding to TRAF6 and regulating its ubiquitin ligase activity which is key in transmitting signals inside the cell.

Biological function summary

TRAF6BP/TAX1BP1 regulates the immune response through its interaction with TRAF6. It plays a part in the formation of a protein complex that influences the balance between pro-inflammatory and anti-inflammatory signals. TRAF6BP affects autophagy an important process for cellular homeostasis and pathogen elimination. TRAF6BP also manages cell survival and apoptosis through its role in signaling cascades.

Pathways

TRAF6BP/TAX1BP1 is heavily involved in the NF-kB and JNK signaling pathways which are pivotal for immune and inflammatory responses. TRAF6BP interacts with proteins like TRAF6 and TAK1 within these pathways to mediate downstream effects that lead to cytokine production and stress responses. These pathways play large roles in maintaining the immune system's balance.

TRAF6BP/TAX1BP1 has connections with inflammatory conditions and certain cancers. It associates with chronic inflammatory diseases due to its role in regulating cytokine production. Changes in TRAF6BP expression or function can lead to unregulated inflammation which is characteristic of rheumatoid arthritis. Furthermore TRAF6BP's regulatory effect on the NF-kB pathway links it to cancer development particularly in cases where overactive NF-kB signaling promotes tumor growth. Its interaction with TRAF6 is critical in these disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation (PubMed : 29940186, PubMed : 30459273, PubMed : 30909570). Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates (PubMed : 17703191). Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production (PubMed : 21885437). Recruits also A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways (PubMed : 17703191). Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling (PubMed : 27736772). As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis (PubMed : 26451915). Upon NBR1 recruitment to the SQSTM1-ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation (PubMed : 33226137, PubMed : 34471133). Mediates the autophagic degradation of other substrates including TICAM1 (PubMed : 28898289).

See full target information TAX1BP1

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

BMC pulmonary medicine 24:542 PubMed39472837

2024

TRIM11 Prevents Ferroptosis in model of asthma by UBE2N-TAX1BP1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Na Li,Guoqing Qiu,Xiangqin Xu,Yan Shen,Yuming Chen

Autophagy 21:160-177 PubMed39193925

2024

Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse White,Young Bong Choi,Jiawen Zhang,Mai Tram Vo,Chaoxia He,Kashif Shaikh,Edward W Harhaj

Nature communications 14:5945 PubMed37741832

2023

Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Taraborrelli,Yasin Şenbabaoğlu,Lifen Wang,Junghyun Lim,Kerrigan Blake,Noelyn Kljavin,Sarah Gierke,Alexis Scherl,James Ziai,Erin McNamara,Mark Owyong,Shilpa Rao,Aslihan Karabacak Calviello,Daniel Oreper,Suchit Jhunjhunwala,Guillem Argiles,Johanna Bendell,Tae Won Kim,Fortunato Ciardiello,Matthew J Wongchenko,Frederic J de Sauvage,Felipe de Sousa E Melo,Yibing Yan,Nathaniel R West,Aditya Murthy

The Journal of biological chemistry 299:104701 PubMed37059186

2023

Oxygen modulates iron homeostasis by switching iron sensing of NCOA4.

Applications

Unspecified application

Species

Unspecified reactive species

Sota Kuno,Kazuhiro Iwai

Cell reports 42:112037 PubMed36701233

2023

The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth R Gallagher,Erika L F Holzbaur

Science (New York, N.Y.) 378:1201-1207 PubMed36520901

2022

ATG9A prevents TNF cytotoxicity by an unconventional lysosomal targeting pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jon Huyghe,Dario Priem,Lisette Van Hove,Barbara Gilbert,Jürgen Fritsch,Yasuo Uchiyama,Esther Hoste,Geert van Loo,Mathieu J M Bertrand

Autophagy 19:805-821 PubMed35920704

2022

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tai-Mei Zhang,Li Liao,Shao-Ying Yang,Min-Ying Huang,Yin-Ling Zhang,Ling Deng,Shu-Yuan Hu,Fan Yang,Fang-Lin Zhang,Zhi-Min Shao,Da-Qiang Li

EMBO reports 23:e54278 PubMed35318808

2022

Iron-induced NCOA4 condensation regulates ferritin fate and iron homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sota Kuno,Hiroaki Fujita,Yu-Ki Tanaka,Yasumitsu Ogra,Kazuhiro Iwai

Autophagy 17:2766-2782 PubMed33143524

2020

Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Zhao Liu,Chun-Gu Hong,Wen-Bao Hu,Meng-Lu Chen,Ran Duan,Hong-Ming Li,Tao Yue,Jia Cao,Zhen-Xing Wang,Chun-Yuan Chen,Xiong-Ke Hu,Ben Wu,Hao-Ming Liu,Yi-Juan Tan,Jiang-Hua Liu,Zhong-Wei Luo,Yan Zhang,Shan-Shan Rao,Ming-Jie Luo,Hao Yin,Yi-Yi Wang,Kun Xia,Lang Xu,Si-Yuan Tang,Rong-Gui Hu,Hui Xie

eLife 8: PubMed31287416

2019

Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Junghyun Lim,Hyunjoo Park,Jason Heisler,Timurs Maculins,Merone Roose-Girma,Min Xu,Brent Mckenzie,Menno van Lookeren Campagne,Kim Newton,Aditya Murthy

View all publications

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重组Anti-TRAF6BP/TAX1BP1抗体[EPR13287(B)] - C-terminal