AB238935

重组Anti-TRAF6BP/TAX1BP1抗体[EPR13287(B)] - BSA and Azide free

Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
了解详情

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(1 Publication)

Knockout Tested Rabbit Recombinant Monoclonal TRAF6BP/TAX1BP1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

T6BP, PRO0105, TAX1BP1, Tax1-binding protein 1, TRAF6-binding protein

1 Images

Lab

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - BSA and Azide free (AB238935)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176572). Anti-TAX1BP1 antibody [EPR13287(B)] (ab176572) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176572 was shown to bind specifically to TAX1BP1. A band was observed at 95 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAX1BP1 knockout cell line. To generate this image, wild-type and TAX1BP1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (<a href='/products/primary-antibodies/traf6bp-tax1bp1-antibody-epr13287b-c-terminal-ab176572'>ab176572</a>) at 1/2000 dilution

Lanes 1 - 2:

Western blot at 20 µg

Lane 2:

Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (<a href='/products/cell-lysates/human-tax1bp1-traf6bp-knockout-hek-293t-cell-lysate-ab257726'>ab257726</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 91 kDa

Observed band size: 95 kDa

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不同偶联物与剂型 (7)

Unconjugated

Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal
578 PE

PE Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal
660 APC

APC Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] – C–terminal

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13287(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab238935 is the carrier-free version of ab176572.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRAF6BP also known as TAX1BP1 is a cytoplasmic protein that participates in important signaling pathways. It has an approximately 75 kDa molecular weight and mainly expresses in immune-related cells including macrophages and lymphocytes. TRAF6BP works as an adaptor protein binding to TRAF6 and regulating its ubiquitin ligase activity which is key in transmitting signals inside the cell.

Biological function summary

TRAF6BP/TAX1BP1 regulates the immune response through its interaction with TRAF6. It plays a part in the formation of a protein complex that influences the balance between pro-inflammatory and anti-inflammatory signals. TRAF6BP affects autophagy an important process for cellular homeostasis and pathogen elimination. TRAF6BP also manages cell survival and apoptosis through its role in signaling cascades.

Pathways

TRAF6BP/TAX1BP1 is heavily involved in the NF-kB and JNK signaling pathways which are pivotal for immune and inflammatory responses. TRAF6BP interacts with proteins like TRAF6 and TAK1 within these pathways to mediate downstream effects that lead to cytokine production and stress responses. These pathways play large roles in maintaining the immune system's balance.

TRAF6BP/TAX1BP1 has connections with inflammatory conditions and certain cancers. It associates with chronic inflammatory diseases due to its role in regulating cytokine production. Changes in TRAF6BP expression or function can lead to unregulated inflammation which is characteristic of rheumatoid arthritis. Furthermore TRAF6BP's regulatory effect on the NF-kB pathway links it to cancer development particularly in cases where overactive NF-kB signaling promotes tumor growth. Its interaction with TRAF6 is critical in these disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation (PubMed : 29940186, PubMed : 30459273, PubMed : 30909570). Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates (PubMed : 17703191). Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production (PubMed : 21885437). Recruits also A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways (PubMed : 17703191). Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling (PubMed : 27736772). As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis (PubMed : 26451915). Upon NBR1 recruitment to the SQSTM1-ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation (PubMed : 33226137, PubMed : 34471133). Mediates the autophagic degradation of other substrates including TICAM1 (PubMed : 28898289).

See full target information TAX1BP1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan

View all publications

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重组Anti-TRAF6BP/TAX1BP1抗体[EPR13287(B)] - BSA and Azide free