Anti-TRADD抗体(ab223040)
Key features and details
- Rabbit polyclonal to TRADD
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TRADD抗体
参阅全部 TRADD 一抗 -
描述
兔多克隆抗体to TRADD -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human TRADD aa 1-312.
Database link: Q15628 -
阳性对照
- WB: K562 cell lysate; Rat kidney tissue lysate. IHC-P: Human liver and tonsil tissues. ICC/IF: U251 cells. IP: K562 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223040于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/200 - 1/2000.
|
|
| WB |
1/500 - 1/5000. Predicted molecular weight: 28,35 kDa.
|
|
| IHC-P |
1/20 - 1/200.
|
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| ICC/IF |
1/50 - 1/200.
|
| 说明 |
|---|
|
IP
1/200 - 1/2000. |
|
WB
1/500 - 1/5000. Predicted molecular weight: 28,35 kDa. |
|
IHC-P
1/20 - 1/200. |
|
ICC/IF
1/50 - 1/200. |
靶标
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功能
Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. -
组织特异性
Found in all examined tissues. -
序列相似性
Contains 1 death domain. -
结构域
Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 8717 Human
- Entrez Gene: 246756 Rat
- Omim: 603500 Human
- SwissProt: Q15628 Human
- Unigene: 460996 Human
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别名
- 9130005N23Rik antibody
- AA930854 antibody
- TNFR1 associated DEATH domain protein antibody
see all
图片
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Western blot - Anti-TRADD antibody (ab223040)All lanes : Anti-TRADD antibody (ab223040) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Rat kidney tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28,35 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (ab223040)Paraffin-embedded human liver tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (ab223040)Paraffin-embedded human tonsil tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody (ab223040)U251 cells stained for TRADD (green) using ab223040 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitation - Anti-TRADD antibody (ab223040)TRADD was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate with ab223040 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab223040 at 1/500 dilution. HRP-conjugated Protein G antibody was used as secondary antibody at 1/2000 dilution.
Lane 1: Rabbit control IgG instead of ab223040 in K562 whole cell lysate.
Lane 2: ab223040 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 10 µg (Input).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223040 尚未被引用在任何文献中。