Anti-TRA-1-60 (R)抗体[TRA-1-60] (ab16288)
![Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-24939-anti-tra-1-60-r-antibody-tra-1-60-western-blot.jpg "Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)")
Key features and details
- Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-TRA-1-60 (R)抗体[TRA-1-60] -
描述
小鼠单克隆抗体[TRA-1-60] to TRA-1-60 (R) -
宿主
Mouse -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human
预测可用于: Rabbit
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免疫原
Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.
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阳性对照
- WB: Human embryonic stem cell lysate. ICC: Human embryonic stem cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
克隆
单克隆 -
克隆编号
TRA-1-60 -
骨髓瘤
P3x66Ag.8-Sp2/0 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
1/500.
|
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| WB | (1) |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
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| 说明 |
|---|
|
ICC
1/500. |
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WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa). |
靶标
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相关性
TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010). -
细胞定位
Cell Membrane -
别名
- TRA 1 60 antibody
图片
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Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody. -
![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-24937-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an Abreview submitted by Ms Fiona LewisImmunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1:500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgM, dilution 1:100, was used as secondary antibody.
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![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-470577-anti-tra-1-60-r-antibody-tra-1-60-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an anonymous collaborator.This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
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![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-272266-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, GermanyImmunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1:500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1:500 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (138)
ab16288 被引用在 138 文献中.
- Kawatani K et al. ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism. Mol Psychiatry 29:809-819 (2024). PubMed: 38135757
- Gopurappilly R et al. Generation of feeder-independent transgene-free iPSC lines from a young-onset Parkinson's disease (YOPD) patient with a homozygous PLA2G6: c.2222G>A (p. Arg741Gln) mutation (NCBSi003-A) and unaffected heterozygous parent (NCBSi004-A). Stem Cell Res 67:103033 (2023). PubMed: 36706537
- Tan Y et al. Engineering a conduction-consistent cardiac patch with graphene oxide modified butterfly wings and human pluripotent stem cell-derived cardiomyocytes. Bioeng Transl Med 8:e10522 (2023). PubMed: 37206241
- Liu X et al. Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa. Elife 12:N/A (2023). PubMed: 37272616
- Qiu JJ et al. Single-cell RNA sequencing of neural stem cells derived from human trisomic iPSCs reveals the abnormalities during neural differentiation of Down syndrome. Front Mol Neurosci 16:1137123 (2023). PubMed: 37396785