AB109301

重组Anti-TR4抗体[EPR1773(2)]

Anti-TR4 antibody [EPR1773(2)]

RabMAb
Recombinant
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(14 Publications)

Rabbit Recombinant Monoclonal TR4 antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 14 publications.

查看别名

TAK1, TR4, NR2C2, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4

7 Images

Unknown

Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (AB109301)

ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)

Predicted band size: 65 kDa

false

Lab

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Lanes 1-3 : Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266228 (CRISPR/Cas9 edited cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NR2C2 CRISPR/Cas9 edited HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NR2C2 (TR4) knockout HEK-293T cell line (<a href='/products/cell-lines/human-nr2c2-tr4-knockout-hek-293t-cell-line-ab266228'>ab266228</a>)

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 65 kDa

Observed band size: 67 kDa

false

Lab

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution

All lanes:

HEK-293 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 67 kDa

false

Lab

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Lanes 1-3 : Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line ab266228 (knockout cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NR2C2 knockout HEK293T cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 65 kDa

Observed band size: 67 kDa

false

Unknown

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

All lanes:

Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution

Lane 1:

PC3 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Predicted band size: 12 kDa,134 kDa,14 kDa,37 kDa,52 kDa,55 kDa,65 kDa,96 kDa

Observed band size: 14 kDa,67 kDa

false

Lab

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution

Lane 1:

PC-12 whole cell lysate at 20 µg

Lane 2:

3T3-L1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 67 kDa

false

Lab

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

All lanes:

Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution

All lanes:

PC-3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 67 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1773(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TR4 also known as testicular receptor 4 or NR2C2 is a nuclear receptor that belongs to the steroid hormone receptor superfamily. It acts as a transcription factor regulating the expression of specific genes. TR4 has a molecular weight of approximately 68 kDa. We find TR4 expression in various tissues including the testis prostate and kidney reflecting its diverse roles in different cellular processes.

Biological function summary

TR4 significantly influences reproductive development and metabolic processes. It plays an important role in regulating genes involved in lipid and glucose metabolism. TR4 does not function alone; it often forms complexes with other proteins to exert its effects on gene expression. The formation of these complexes enhances its ability to control a wide range of biological activities.

Pathways

TR4 actively participates in the insulin signaling and androgen receptor pathways. It modulates the action of insulin by influencing genes associated with glucose metabolism impacting energy homeostasis. In the androgen receptor pathway TR4 shares regulatory roles with proteins like AR and NR2C1 ensuring proper development and function of reproductive organs. Its involvement in these pathways exemplifies its role in maintaining metabolic and reproductive health.

Scientists link TR4 with metabolic syndrome and prostate cancer. Abnormal TR4 expression or function associates with disrupted glucose and lipid metabolism contributing to metabolic syndrome. In prostate cancer TR4 interacts with the androgen receptor affecting tumor growth and progression. Understanding these connections helps in exploring TR4 as a potential therapeutic target for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.

See full target information NR2C2

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

The Korean journal of pain 38:292-307 PubMed40556327

2025

MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation targeting MyD88 in bone cancer pain.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Liu,Denggui Wang,Zhirong Yan,Min Zhou

Frontiers in immunology 15:1386780 PubMed38756773

2024

TAK1 inhibition mitigates intracerebral hemorrhage-induced brain injury through reduction of oxidative stress and neuronal pyroptosis via the NRF2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Chunli Chen,Lite Ge,Zheng Jiang,Zhiping Hu,Lihong Yin

European journal of medical research 29:151 PubMed38429762

2024

TR4 worsen urosepsis by regulating GSDMD.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Shibin Zhu,Zhenwei Zhou,Zhenghui Wang,Wei Zhuang,Dingwei Xue,Zeyi Lu,Qiming Zheng,Lifeng Ding,Liangliang Ren,Wenqing Luo,Ruyue Wang,Guangju Ge,Liqun Xia,Gonghui Li,Haiyang Wu

Nature communications 14:1732 PubMed36977669

2023

A regulatory variant at 19p13.3 is associated with primary biliary cholangitis risk and ARID3A expression.

Applications

Unspecified application

Species

Unspecified reactive species

You Li,Zhiqiang Li,Ruiling Chen,Min Lian,Hanxiao Wang,Yiran Wei,Zhengrui You,Jun Zhang,Bo Li,Yikang Li,Bingyuan Huang,Yong Chen,Qiaoyan Liu,Zhuwan Lyu,Xueying Liang,Qi Miao,Xiao Xiao,Qixia Wang,Jingyuan Fang,YongYong Shi,Xiangdong Liu,Michael F Seldin,M Eric Gershwin,Ruqi Tang,Xiong Ma

Frontiers in molecular biosciences 8:670409 PubMed34616769

2021

Testicular Nuclear Receptor 4 Regulates Proliferation and Apoptosis of Bladder Cancer Bcl-2.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Wenqin Luo,Xuliang Wang,Dingwei Xue,Liangliang Ren,Li Xu,Guangju Ge,Liqun Xia,Shicheng Yu,Mingchao Wang,Zhenwei Zhou,Gonghui Li,Haiyang Wu

Journal of cellular and molecular medicine 25:2404-2417 PubMed33491272

2021

Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liqun Xia,Danyang Shen,Youyun Zhang,Jieyang Lu,Mingchao Wang,Huan Wang,Yuanlei Chen,Dingwei Xue,Dajiang Xie,Gonghui Li

Journal of assisted reproduction and genetics : PubMed33047186

2020

Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vila-Cejudo,Sandra Alonso-Alonso,Anna Pujol,Josep Santaló,Elena Ibáñez

The Journal of clinical endocrinology and metabolism 106:e232-e246 PubMed33000123

2020

Targeting Corticotroph HDAC and PI3-Kinase in Cushing Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyun Zhang,Robert Damoiseaux,Lilit Babayan,Everett Kanediel Rivera-Meza,Yingying Yang,Marvin Bergsneider,Marilene B Wang,William H Yong,Kathleen Kelly,Anthony P Heaney

Oncogene 39:1891-1903 PubMed31748715

2019

Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Linyi Hu,Yin Sun,Jie Luo,Xiang He,Meihua Ye,Gonghui Li,Yong Zhang,Jian Bai,Dahong Zhang,Chawnshang Chang

Oncogene 39:530-545 PubMed31501521

2019

Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hangchuan Shi,Yin Sun,Miao He,Xiong Yang,Michiaki Hamada,Tsukasa Fukunaga,Xiaoping Zhang,Chawnshang Chang

View all publications

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-TR4抗体[EPR1773(2)]

Anti-TR4 antibody [EPR1773(2)]

Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

Western blot - Anti-TR4 antibody [EPR1773(2)] (AB109301)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原