重组Anti-TPT1L + TCTP抗体[EPR13882(B)] (ab182557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13882(B)] to TPT1L + TCTP
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TPT1L + TCTP抗体[EPR13882(B)]
参阅全部 TPT1L + TCTP 一抗 -
描述
兔单克隆抗体[EPR13882(B)] to TPT1L + TCTP -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, 293 and HepG2 cell lysates; Human prostate tissue; Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13882(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/240.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 16, 20 kDa).
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IP |
1/40 - 1/60.
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说明 |
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Flow Cyt (Intra)
1/240. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 16, 20 kDa). |
IP
1/40 - 1/60. |
图片
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All lanes : Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 16, 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling FLJ44635 + TCTP with ab182557 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling FLJ44635 + TCTP with ab182557 at 1/240 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182557 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
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