重组Anti-TPPP抗体[EPR3315] (ab119987)
![Western blot - Anti-TPPP antibody [EPR3315] (ab119987)](https://www.abcam.cn/ps/products/119/ab119987/Images/ab119987-382816-anti-tppp-antibody-epr3315-westernblot-brain-human.jpg "Western blot - Anti-TPPP antibody [EPR3315] (ab119987)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3315] to TPPP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-TPPP抗体[EPR3315]
参阅全部 TPPP 一抗 -
描述
兔单克隆抗体[EPR3315] to TPPP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human fetal brain and SH-SY5Y cell lysates; Human astrocytoma tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3315 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119987于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
靶标
-
功能
May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the TPPP family. -
翻译后修饰
Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity. -
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies. - Information by UniProt
-
数据库链接
- Entrez Gene: 11076 Human
- Omim: 608773 Human
- SwissProt: O94811 Human
- Unigene: 481466 Human
-
别名
- 25 kDa brain specific protein antibody
- 25 kDa brain-specific protein antibody
- Brain specific protein p25 alpha antibody
see all
图片
-
Western blot - Anti-TPPP antibody [EPR3315] (ab119987)Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution (Purified) + Human brain lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
-
![Western blot - Anti-TPPP antibody [EPR3315] (ab119987)](https://www.abcam.cn/ps/products/119/ab119987/Images/ab119987-133585-anti-tppp-antibody-epr3315-western-blot.jpg)
Western blot - Anti-TPPP antibody [EPR3315] (ab119987)All lanes : Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDaThe cell lysates were prepared in 1%SDS Hot lysis method
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (ab119987)](https://www.abcam.cn/ps/products/119/ab119987/Images/ab119987-133584-anti-tppp-antibody-epr3315-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPPP antibody [EPR3315] (ab119987)ab119987 at 1/100 dilution staining TPPP in Paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-TPPP antibody [EPR3315] (ab119987)](https://www.abcam.cn/ps/products/119/ab119987/Images/ab119987-4-benefits-of-recombinant-antibodies.png)
Anti-TPPP antibody [EPR3315] (ab119987)
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab119987 尚未被引用在任何文献中。