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Anti-TPN抗体(ab13518)

Reviews (1)Q&A (1)References (7)

Key features and details

  • Rabbit polyclonal to TPN
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-TPN antibody [7F6] (ab252869)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-TPN抗体
    参阅全部 TPN 一抗

  • 描述

    兔多克隆抗体to TPN

  • 宿主

    Rabbit

  • 特异性

    Weakly cross-reacts with bovine Tapasin.

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Cow

  • 免疫原

    Synthetic peptide corresponding to Human TPN aa 430-448.
    Sequence:

    LGWAAVYLSTCKDSKKKAE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab13518于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

  • 组织特异性

    Neutrophils, mostly in fully differentiated cells.

  • 序列相似性

    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 结构域

    The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.

  • 细胞定位

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • NGS 17 antibody
      • NGS-17 antibody
      • NGS17 antibody
      • TAP associated protein antibody
      • TAP binding protein antibody
      • TAP binding protein (tapasin) antibody
      • TAP BP antibody
      • TAP-associated protein antibody
      • TAP-binding protein antibody
      • TAPA antibody
      • Tapasin antibody
      • TAPBP antibody
      • TPN antibody
      • TPSN antibody
      • TPSN_HUMAN antibody
      see all

    文献 (7)

    发表研究结果有使用 ab13518？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab13518 被引用在 7 文献中.

    • Domnick A  et al. Molecular basis of MHC I quality control in the peptide loading complex. Nat Commun 13:4701 (2022). PubMed: 35948544
    • Wang KW  et al. An antigen processing and presentation signature for prognostic evaluation and immunotherapy selection in advanced gastric cancer. Front Immunol 13:992060 (2022). PubMed: 36311733
    • Liang YH  et al. Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing. Sci Rep 11:9080 (2021). PubMed: 33907276
    • Lazaridou MF  et al. Identification of miR-200a-5p targeting the peptide transporter TAP1 and its association with the clinical outcome of melanoma patients. Oncoimmunology 9:1774323 (2020). PubMed: 32923135
    • Wynne JW  et al. Characterization of the Antigen Processing Machinery and Endogenous Peptide Presentation of a Bat MHC Class I Molecule. J Immunol 196:4468-76 (2016). PubMed: 27183594
    • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). PubMed: 24971583
    • Chapiro J  et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . PubMed: 16393993

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TPN antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Fibroblast cell line)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    100 µg
    Specification
    Human Fibroblast cell line
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2012

    Question

    Our end user sent claim report again. He want to receive credit. Please check this claim report and figure and let me know how to I take care. Claim Report Product name Anti-tapasin ab13518 Batch no: 65676 * Research Purpose Immunoprecipitation 1. Antibody Storage Conditions (temperature/reconstitution etc) -20 C 2. Description of the problem (high background, wrong band size, more bands, no band etc.) No band 3. Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) HeLa cell hole lysate 4. Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) 1 % triton X-100 in PBS 5. Amount of protein loaded 500 ug 6. Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) 10 % SDS PAGE gel in reducing condition 7. Transfer and blocking conditions (Buffer/Time period, Blocking agent etc.) General transfer buffer / 90 volt / 1 hour / PBS-T containing 5 % skim milk 8. Primary antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) immunoblot à [a competitor] / goat / 1:800 / 12 hours / three times for 10 min (PBS-T) 9. Secondary antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) [a competitor] / anti-goat / 1:5000 / 1 hour / three times for 15 min (PBS-T) 10. Detection method (ECL, ECL Plus etc.) Pierce, super signal 11. Positive and negative controls used (Please specify) Goat anti-tapasin 12. How many times have you tried the Western? Three times 13. Have you run a “No Primary” control? (Yes or No) No 14. Do you obtain the same results every time? (Yes or No) Yes 15. What steps have you altered? Dilution / incubation time 16. Additional Notes Control : [a competitor] antibody

    Read More
    Answer

    I'm sorry to hear your customer is having problems with ab13518 and thank him for taking the time to give us protocol details. We have not received any complaints regarding this antibody and are confused by the complaint form: the customer mentions immunoprecipitation as the application used for this antibody. However, from the protocol it appears that the immunoprecipitation step was done with the competitor antibody and no details for the Ip were provided. If this is correct we suggest testing our antibody on lysates of HeLa cells by western blotting. A 1:1,000 dilution of ab13518 is sufficent for detection of Tapasin in 20µg of HeLa cell lysate and will determine whether the antibody is faulty. Thank you for sending us an image, however it is unclear to us what it shows as there is no legend. Is it comparing two immunoprecipitations with different antibodies and reprobe with the same antibody? We would like to emphasise to use an adequate lysis buffer (e.g RIPA buffer) with a cocktail of protease inhibitors to prevent the degradation of the protein rather than PBST. Samples should be mixed with loading buffer (containing SDS and betamercaptoethanol) and boiled for 5min at 95C to unfold and denature the protein for adequate recognition. As this information was not present on the form we wish to confirm this recommendation. I apologise for asking clarification of the problem. We have updated our questionnaires for our distributors and can send you those so that we can obtain the maximum information from the customer initially and help the customer as quickly as possible (for example we have an immunoprecipitation specific questionnaire). Please let us know which e-mail address you would like the forms to be sent to you and I will send those immediately. Thank you for your patience, we look forward to hearing from you,

    Read More

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