AB249390

重组Anti-TPMT抗体[EPR10821(B)] - BSA and Azide free

Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal TPMT antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

查看别名

Thiopurine S-methyltransferase, Thiopurine methyltransferase, TPMT

3 Images

Flow Cytometry (Intracellular) - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (AB249390)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (AB249390)

This data was developed using ab166909, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells using ab166909 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (AB249390)

This data was developed using ab166909, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

TF-1 cell lysate at 10 µg

Predicted band size: 28 kDa

false

Lab

Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (AB249390)

This data was developed using ab166909, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab166909 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab166909 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. ab166909 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TPMT antibody [EPR10821(B)] (<a href='/products/primary-antibodies/tpmt-antibody-epr10821b-ab166909'>ab166909</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TPMT knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

K562 whole cell lysate at 20 µg

Predicted band size: 28 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-TPMT antibody [EPR10821(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10821(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

ab249390 is the carrier-free version of ab166909.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Thiopurine S-methyltransferase often called TPMT plays a role as an enzyme that catalyzes the S-methylation of thiopurine compounds like 6-mercaptopurine and azathioprine. It exists as a monomer with a molecular mass of about 28 kDa. In humans TPMT expresses mainly in the liver and kidney. The activity of TPMT varies among individuals due to genetic polymorphisms.

Biological function summary

TPMT is important in the metabolism of therapeutic thiopurine drugs. These drugs often require precise dosing to achieve effectiveness without toxicity. TPMT does not form part of a larger complex; it functions independently to methylate thiopurine drugs. Genetic variations can lead to different TPMT activity levels affecting a person's response to these drugs significantly.

Pathways

TPMT is integral to the drug metabolism pathway specifically in the detoxification and elimination processes of thiopurine drugs. While TPMT predominantly acts on thiopurines thioredoxin reductase 1 (TXNRD1) may also have a role in modulating redox balance and affect the thiopurine metabolism indirectly through related cellular processes. Together they influence the cellular response to drugs like azathioprine.

Genetic variants in TPMT significantly contribute to thiopurine drug intolerance or toxicity particularly in conditions like inflammatory bowel disease and acute lymphoblastic leukemia where these drugs are used. Patients with low TPMT activity are at risk for severe myelosuppression a life-threatening bone marrow suppression. TPMT's relationship with the enzyme inosine monophosphate dehydrogenase (IMPDH) also illustrates its connection to nucleotide synthesis further linking TPMT to therapeutic outcomes in cancers.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor (PubMed : 18484748, PubMed : 657528). TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.

See full target information TPMT

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-TPMT抗体[EPR10821(B)] - BSA and Azide free