AB243028

Anti-Tpit 抗体 [CL6251]

Name: Tpit抗体[CL6251] (ab243028)| Abcam中文官网
Brand: Abcam Limited
SKU: AB243028
Price: 5644 CNY
Availability: InStock

Anti-Tpit antibody [CL6251]

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(7 Publications)

Anti-Tpit antibody [CL6251] (ab243028) is a mouse monoclonal antibody detecting Tpit in IHC-P. Suitable for Human.

查看别名

TPIT, TBX19, T-box transcription factor TBX19, T-box protein 19

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Formalin-fixed, paraffin-embedded human pituitary gland tissue stained for Tpit with ab243028 at a 1 : 1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Negative control - Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tpit with ab243028 at a 1 : 1000 dilution in immunohistochemical analysis.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CL6251

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

Recombinant Fragment Protein within Human TBX19 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60806

表位

Binds to an epitope located within the peptide sequence VLGEPSLTSIAVSTW as determined by overlapping synthetic peptides

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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产品详情

What is this antibody validated in?
Anti-Tpit antibody [CL6251] (ab243028) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Purified from TCS.

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tpit also known as TPIT or Tbx19 is a transcription factor that plays a significant role in the differentiation of certain pituitary cells. It has a molecular mass of approximately 62 kDa. Tpit is expressed in the pituitary gland specifically in the cells that produce pro-opiomelanocortin (POMC) which develop into corticotrophs and melanotrophs. Its expression is important for the proper functioning of these endocrine cells directly impacting the hormone production process.

Biological function summary

Tpit acts as a regulator in pituitary development and functioning. It binds to DNA and activates the transcription of POMC leading to the production of hormones such as ACTH (adrenocorticotropic hormone) and MSH (melanocyte-stimulating hormone). Tpit is not a part of a larger complex but functions independently to exert its transcriptional regulation role. Through this mechanism Tpit influences metabolic and stress-related processes in the body.

Pathways

Tpit is deeply involved in the hypothalamic-pituitary-adrenal (HPA) axis a critical stress response system that regulates cortisol production. Tpit regulates POMC expression which in turn affects downstream hormones like ACTH integral to the HPA axis. Moreover its activity is related to other proteins such as CRH (corticotropin-releasing hormone receptor) as they jointly control hormonal signaling pathways. Tpit ensures that the pituitary gland properly synthesizes and secretes hormones in response to various physiological stimuli.

Tpit mutations are associated with disorders such as isolated ACTH deficiency. This disorder results from the failure to produce ACTH leading to adrenal insufficiency and symptoms of fatigue and hypoglycemia. Tpit's role links it to other proteins such as NR5A1 (nuclear receptor subfamily 5 group A member 1) that influence pituitary function and development. Understanding Tpit's involvement in these disorders can help in the development of targeted therapies and improved diagnostic methods in endocrine diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional regulator involved in developmental processes. Can activate POMC gene expression and repress the alpha glycoprotein subunit and thyroid-stimulating hormone beta promoters.

See full target information TBX19

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Pituitary 26:227-236 PubMed36952069

2023

TGFBR3L is associated with gonadotropin production in non-functioning gonadotroph pituitary neuroendocrine tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Anders Jensen Kolnes,Kristin Astrid Berland Øystese,Evelina Sjöstedt,Nicoleta Cristina Olarescu,Ansgar Heck,Jens Pahnke,Daniel Dahlberg,Jon Berg-Johnsen,Geir Ringstad,Olivera Casar-Borota,Jens Bollerslev,Anders Palmstrøm Jørgensen

Cells 11: PubMed35954244

2022

CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Dong,Wenjian Shi,Yongliang Liu,Jingwu Li,Yu Zhang,Guilan Dong,Xiaoliu Dong,Hua Gao

Acta histochemica et cytochemica 54:87-96 PubMed34276102

2021

Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT.

Applications

Unspecified application

Species

Unspecified reactive species

Hiromi Onizuka,Kenta Masui,Kosaku Amano,Takakazu Kawamata,Tomoko Yamamoto,Yoji Nagashima,Noriyuki Shibata

Aging 13:11281-11295 PubMed33820867

2021

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Xu,Fei Zhang,Junlan Lu,Kexin Wang,Peng Pan,Ye Sun,Yuxin Zhang

Genes 11: PubMed33261069

2020

Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from - and -Cell Lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Taniguchi-Ponciano,Eduardo Peña-Martínez,Gloria Silva-Román,Sandra Vela-Patiño,Ana Laura Guzman-Ortiz,Hector Quezada,Erick Gomez-Apo,Laura Chavez-Macias,Sophia Mercado-Medrez,Guadalupe Vargas-Ortega,Ana Laura Espinosa-de-Los-Monteros,Baldomero Gonzales-Virla,Aldo Ferreira-Hermosillo,Etual Espinosa-Cardenas,Claudia Ramirez-Renteria,Ernesto Sosa,Blas Lopez-Felix,Gerardo Guinto,Daniel Marrero-Rodríguez,Moises Mercado

Scientific reports 10:19373 PubMed33168897

2020

Transcriptome and methylome analysis reveals three cellular origins of pituitary tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Taniguchi-Ponciano,Sergio Andonegui-Elguera,Eduardo Peña-Martínez,Gloria Silva-Román,Sandra Vela-Patiño,Erick Gomez-Apo,Laura Chavez-Macias,Guadalupe Vargas-Ortega,Laura Espinosa-de-Los-Monteros,Baldomero Gonzalez-Virla,Carolina Perez,Aldo Ferreira-Hermosillo,Etual Espinosa-Cardenas,Claudia Ramirez-Renteria,Ernesto Sosa,Blas Lopez-Felix,Gerardo Guinto,Daniel Marrero-Rodríguez,Moises Mercado

Cancers 11: PubMed31835737

2019

How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?

Applications

Unspecified application

Species

Unspecified reactive species

María Eugenia Torregrosa-Quesada,Araceli García-Martínez,Sandra Silva-Ortega,Sebastián Martínez-López,Rosa Cámara,Carmen Fajardo,Cristina Lamas,Ignacio Aranda,Antonio Picó

View all publications

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Anti-Tpit 抗体 [CL6251]

Anti-Tpit antibody [CL6251]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

表位