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重组RabMAb

重组Anti-Torsin A/DYT1抗体[EP2569Y] (ab76133)

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Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
  • Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
  • Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2569Y] to Torsin A/DYT1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Torsin A/DYT1抗体[EP2569Y]
    参阅全部 Torsin A/DYT1 一抗

  • 描述

    兔单克隆抗体[EP2569Y] to Torsin A/DYT1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Torsin A/DYT1 aa 300-400 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • WB: SW480, Caco-2, A549, NIH/3T3, and C6 whole cell lysates. Rat brain and mouse lung tissue lysates; Flow Cyt (intra): SH-SY5Y cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76133于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.

For unpurified used at 1/150  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
说明
Flow Cyt (Intra)
1/100.

For unpurified used at 1/150  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

靶标

图片

  • Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    All lanes : Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133) at 1/5000 dilution (purified)

    Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 6 : Mouse lung lysate
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)

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