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Anti-TOR1AIP1抗体[RL13] (ab2737)

Submit a review Submit a question References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)
  • Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [RL13] (ab2737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)

Key features and details

  • Mouse monoclonal [RL13] to TOR1AIP1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

概述

  • 产品名称

    Anti-TOR1AIP1抗体[RL13]
    参阅全部 TOR1AIP1 一抗

  • 描述

    小鼠单克隆抗体[RL13] to TOR1AIP1

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Full length protein corresponding to Rat TOR1AIP1. This is a Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

  • 阳性对照

    • IHC-P: Rat lymph node, breast, colon tissue. ICC: NS-1 cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2737于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/20 - 1/50.
ICC/IF
1/100.
说明
IHC-P
1/20 - 1/50.
ICC/IF
1/100.

靶标

  • 功能

    Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.

  • 序列相似性

    Belongs to the TOR1AIP family.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus inner membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Lamin-associated protein 1B antibody
      • Lamina associated polypeptide 1B antibody
      • Lamina associated polypeptide 1C antibody
      • LAP1 antibody
      • LAP1B antibody
      • TOIP1_HUMAN antibody
      • TOR1AIP1 antibody
      • Torsin A interacting protein 1 antibody
      • Torsin-1A-interacting protein 1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)

      Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of rat lymph node tissue labeling TOR1AIP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [RL13] (ab2737)
      Immunocytochemistry/ Immunofluorescence - Anti-TOR1AIP1 antibody [RL13] (ab2737)

      Immunocytochemical analysis of NS-1 (Mouse myeloma cell line) cells labeling TOR1AIP1 with ab2737 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)

      Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) rat breast tissue labeling TOR1AIP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOR1AIP1 antibody [RL13] (ab2737)

      Immunohistochemical analysis of (Formalin/PFA-fixed paraffin-embedded sections) rat colon tissue labeling TOR1AIP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    文献 (6)

    发表研究结果有使用 ab2737？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2737 被引用在 6 文献中.

    • Liao JJ  et al. Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine 1-phosphate G protein-coupled receptors. J Biol Chem 282:1964-72 (2007). WB . PubMed: 17121832
    • Yang L  et al. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J Cell Biol 139:1077-87 (1997). PubMed: 9382857
    • Guan T  et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). PubMed: 8589458
    • Martin L  et al. cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane. J Biol Chem 270:8822-8 (1995). PubMed: 7721789
    • Foisner R & Gerace L Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 73:1267-79 (1993). PubMed: 8324822
    • Senior A & Gerace L Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina. J Cell Biol 107:2029-36 (1988). PubMed: 3058715

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