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AB269765

Anti-Topoisomerase II alpha抗体[TOP2A/1362] - BSA and Azide free

Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free

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Mouse Monoclonal Topoisomerase II alpha antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TOP2A aa 1350-1500.

查看别名

TOP2, TOP2A, DNA topoisomerase 2-alpha

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (AB269765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (AB269765)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Topoisomerase II alpha with ab219321 at 1 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219321)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (AB269765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (AB269765)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Topoisomerase II alpha with ab219321 at 1 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219321)

Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (AB269765)
  • Protein Array

Unknown

Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (AB269765)

ab219321 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219321)

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

TOP2A/1362

亚型

IgG2b

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, Protein Array

applications

免疫原

Recombinant Fragment Protein within Human TOP2A aa 1350-1500. The exact immunogen used to generate this antibody is proprietary information.

P11388

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

产品详情

ab269765 is a carrier free version of ab219321.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
纯化说明
ab219321 was purified from Bioreactor Concentrate by Protein A/G.
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha also known as TOP2A is an enzyme that plays a critical role in DNA replication and cell division. It is a homodimeric protein with a mass of around 170 kDa and primarily localizes in the nucleus. TOP2A catalyzes the transient breaking and rejoining of double-stranded DNA necessary for resolving DNA tangles and supercoils during the replication process. TOP2A is highly expressed in proliferating cells due to its role in cell cycle progression making it a significant target in cancer research.
Biological function summary

TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.

Pathways

TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.

Overexpression of TOP2A is frequently associated with various cancers including breast and prostate cancer. The enzyme's increased activity promotes uncontrolled cell proliferation a hallmark of cancer development. Antitopoisomerase drugs which target this pathway are used in cancer treatments to inhibit TOP2A activity. Additionally mutations or dysregulation of TOP2A-related pathways may also contribute to chemoresistance. Understanding TOP2A interactions and regulation provides insight into its role in cancer and opens avenues for targeted therapeutic interventions.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed : 17567603, PubMed : 18790802, PubMed : 22013166, PubMed : 22323612, PubMed : 27754753). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
See full target information TOP2A

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