重组Anti-Topoisomerase II alpha抗体[EP1102Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

• WB

Lab

Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lysate were prepared using 1%SDS Hot lysis method.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

All lanes:

Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (<a href='/products/primary-antibodies/topoisomerase-ii-alpha-antibody-ep1102y-ab52934'>ab52934</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method 20μg at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 174 kDa

false

Exposure time: 7s

• WB

Lab

Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52934).

Exposure time :
Lanes 1-3 : 1 second.
Lanes 4-7 : 3 minutes.
Lane 8 : 7 seconds.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (<a href='/products/primary-antibodies/topoisomerase-ii-alpha-antibody-ep1102y-ab52934'>ab52934</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma) whole cell lysate at 20 µg

Lane 4:

Human breast cancer lysate at 20 µg

Lane 5:

Mouse thymus lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 7:

Rat thymus lysate at 20 µg

Lane 8:

Rat testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 174 kDa

Observed band size: 174 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (AB181828)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.