Name: Anti-Topoisomerase II alpha antibody [EP1102Y]
Brand: RabMAb
SKU: ab52934
Rating: 4 (1 reviews)

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Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1102Y] to Topoisomerase II alpha
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-Topoisomerase II alpha抗体[EP1102Y]
参阅全部 Topoisomerase II alpha 一抗
描述

兔单克隆抗体[EP1102Y] to Topoisomerase II alpha
宿主

Rabbit
特异性

Please be aware that the antibody provided positive results in cell lysate samples but negative results in some tissue lysate samples.
经测试应用

适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide within Human Topoisomerase II alpha aa 1500-1600 (C terminal). The exact sequence is proprietary.
Database link: P11388
阳性对照
- HeLa whole cell lysate (ab150035); human breast carcinoma tissue. WB: HeLa, Jurkat, Neuro-2a and PC-12 whole cell lysate; Human breast cancer, Mouse thymus lysate, Rat thymus lysate and Rat testis lysate
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
解离常数（K_D）

K_D = 5.39 x 10 ^-10 M
Learn more about K_D
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP1102Y
同种型

IgG
研究领域

Alternative Versions
- Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (ab181828)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Topoisomerase II alpha protein (ab159735)

The Abpromise guarantee

Abpromise™承诺保证使用ab52934于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IHC-P	(1)	Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

说明
WB 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

应用说明

Is unsuitable for Flow Cyt or ICC/IF.

功能

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
序列相似性

Belongs to the type II topoisomerase family.
翻译后修饰

Phosphorylation has no effect on catalytic activity.
细胞定位

Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
Target information above from: UniProt accession P11388 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 7153 Human
- Entrez Gene: 21973 Mouse
- Entrez Gene: 360243 Rat
- Omim: 126430 Human
- SwissProt: P11388 Human
- SwissProt: Q01320 Mouse
- SwissProt: P41516 Rat
- Unigene: 156346 Human
- Unigene: 4237 Mouse
- Unigene: 90996 Rat
see all
别名
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
- DNA topoisomerase (ATP hydrolyzing) antibody
- DNA topoisomerase 2 alpha antibody
- DNA topoisomerase 2-alpha antibody
- DNA topoisomerase II 170 kD antibody
- DNA topoisomerase II alpha isozyme antibody
- DNA topoisomerase II antibody
- DNA Topoisomerase2 antibody
- TOP 2A antibody
- TOP2 antibody
- TOP2A antibody
- TOP2A_HUMAN antibody
- Topoisomerase DNA II alpha 170kDa antibody
- TP2A antibody
see all

Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method 20µg

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 174 kDa

Exposure time: 7 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

All lysate were prepared using 1%SDS Hot lysis method.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma) whole cell lysate
Lane 4 : Human breast cancer lysate
Lane 5 : Mouse thymus lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : Rat thymus lysate
Lane 8 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 174 kDa
Observed band size: 174 kDa

Exposure time:

Lanes 1-3: 1 second.
Lanes 4-7: 3 minutes.
Lane 8: 7 seconds.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
OI-RD Scanning - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Language
Datasheet download

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发表研究结果有使用 ab52934？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52934 被引用在 63 文献中.

Jia X et al. Toosendanin targeting eEF2 impedes Topoisomerase I & II protein translation to suppress esophageal squamous cell carcinoma growth. J Exp Clin Cancer Res 42:97 (2023). PubMed: 37088855
Fu Y et al. Circular RNA Ubiquitin-associated Protein 2 Silencing Suppresses Bladder Cancer Progression by Downregulating DNA Topoisomerase 2-alpha Through Sponging miR-496. Eur Urol Open Sci 50:31-42 (2023). PubMed: 37101770
Park JM et al. Genome-Wide CRISPR Screens Reveal ZATT as a Synthetic Lethal Target of TOP2-Poison Etoposide That Can Act in a TDP2-Independent Pathway. Int J Mol Sci 24:N/A (2023). PubMed: 37047518
Zhang H et al. RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons. Sci Adv 9:eadi6681 (2023). PubMed: 38055811
Dunkley S & Mogessie B Actin limits egg aneuploidies associated with female reproductive aging. Sci Adv 9:eadc9161 (2023). PubMed: 36662854

View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Colon ...)

Specification

Colon ...

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0

Permeabilization

Yes - Wash Buffer from DAKO

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Rudolf Jung

Verified customer

提交于 Mar 18 2011

Question

Testing dicount for ab52934 in ChIP.

Abcam community

Verified customer

Asked on Nov 04 2011

Answer

DISCOUNT CODE: ****** Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab52934 in ChIP]. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for ChIPand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

回复于 Nov 04 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

解离常数（KD）

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (63)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Topoisomerase II alpha antibody [EP1102Y]

解离常数（K_D）