Anti-TopBP1抗体(ab2402)
Key features and details
- Rabbit polyclonal to TopBP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TopBP1抗体
参阅全部 TopBP1 一抗 -
描述
兔多克隆抗体to TopBP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human TopBP1 aa 1400-1500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_008958.1 (GeneID 11073).
Database link: Q92547 -
阳性对照
- WB: HeLa, HEK-293T, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: TopBP1 in HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
靶标
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功能
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. -
组织特异性
Highly expressed in heart, brain, placenta, lung and kidney. -
序列相似性
Contains 8 BRCT domains. -
翻译后修饰
Phosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. -
细胞定位
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 11073 Human
- Entrez Gene: 235559 Mouse
- Omim: 607760 Human
- SwissProt: Q92547 Human
- SwissProt: Q6ZQF0 Mouse
- Unigene: 593379 Human
- Unigene: 259893 Mouse
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别名
- DNA topoisomerase 2 binding protein 1 antibody
- DNA topoisomerase 2-binding protein 1 antibody
- DNA topoisomerase II binding protein 1 antibody
see all
图片
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Western blot - Anti-TopBP1 antibody (ab2402)All lanes : Anti-Cytokeratin 10 antibody [LHP1] (ab2409) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 173.3 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-TopBP1 antibody (ab2402)Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies: ab2409 used for IP at 3 µg per reaction.
Two lots tested (Lanes 1 and 2).
Lane 3 is Control IgG.
For blotting immunoprecipitated TopBP1, ab2409 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-TopBP1 antibody (ab2402)All lanes : Anti-Cytokeratin 10 antibody [LHP1] (ab2409) at 0.1 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 173.3 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
数据表及文件
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SDS download
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Datasheet download
文献 (41)
ab2402 被引用在 41 文献中.
- Trivedi P et al. Mitotic tethering enables inheritance of shattered micronuclear chromosomes. Nature 618:1049-1056 (2023). PubMed: 37316668
- De Marco Zompit M et al. The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis. Nat Commun 13:4143 (2022). PubMed: 35842428
- Bradbury A et al. The Role of ATR Inhibitors in Ovarian Cancer: Investigating Predictive Biomarkers of Response. Cells 11:N/A (2022). PubMed: 35954206
- Balbo Pogliano C et al. The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells. Sci Adv 8:eabk0221 (2022). PubMed: 35119917
- Adam S et al. The CIP2A-TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer. Nat Cancer 2:1357-1371 (2021). PubMed: 35121901