Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-TOMM22/TOM22抗体[EPR13505] - N-terminal (ab179826)

Submit a review Submit a question References (3)
Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
  • Flow Cytometry (Intracellular) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
  • Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
  • Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13505] to TOMM22/TOM22 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TOMM22/TOM22抗体[EPR13505] - N-terminal
    参阅全部 TOMM22/TOM22 一抗

  • 描述

    兔单克隆抗体[EPR13505] to TOMM22/TOM22 - N-terminal

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human TOMM22/TOM22 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NS69

  • 阳性对照

    • HeLa, K562, Raji and 293T cell lysates. Kidney and liver tissue. Raji and 293T cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab179826于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 细胞定位

    Mitochondrion outer membrane; Single-pass membrane protein

  • 数据库链接

    • 别名

      • 1C9 2 antibody
      • hTom 22 antibody
      • hTom22 antibody
      • Mitochondrial import receptor subunit TOM 22 homolog antibody
      • Mitochondrial import receptor subunit TOM22 homolog antibody
      • Mitochondrial import receptor Tom 22 antibody
      • Mitochondrial import receptor Tom22 antibody
      • MST 065 antibody
      • MST065 antibody
      • MSTP 065 antibody
      • MSTP065 antibody
      • TOM 22 antibody
      • TOM22 antibody
      • TOMM 22 antibody
      • TOMM22 antibody
      • Translocase of outer membrane 22 kDa subunit homolog antibody
      • Translocase of outer mitochondrial membrane 22 homolog antibody
      see all

    图片

    • Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      All lanes : Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

      Immunofluorescent analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Flow Cytometry (Intracellular) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

      Intracellular flow cytometric analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/10 (red) or a rabbit IgG (negative) (green). 

       

       

    • Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

      Western blot analysis on immunoprecipitation pellet from Raji cell lysate using ab179826 at 1/10 dilution for IP.

    • Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)
      Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

    文献 (3)

    发表研究结果有使用 ab179826？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab179826 被引用在 3 文献中.

    • Furihata T  et al. Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Commun Biol 4:138 (2021). PubMed: 33514783
    • Alowaidi F  et al. Investigating the role of CRIPTO-1 (TDGF-1) in glioblastoma multiforme U87 cell line. J Cell Biochem N/A:N/A (2018). PubMed: 30426531
    • Pollara J  et al. Circulating mitochondria in deceased organ donors are associated with immune activation and early allograft dysfunction. JCI Insight 3:N/A (2018). PubMed: 30089724

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab179826.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com