Anti-Tollip抗体(ab223763)
Key features and details
- Rabbit polyclonal to Tollip
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Tollip抗体
参阅全部 Tollip 一抗 -
描述
兔多克隆抗体to Tollip -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Tollip aa 100-250.
Database link: Q9H0E2 -
阳性对照
- IHC-P: Human small intestine tissue. WB: RT-4, U-251 MG and NBT-II cell lysates. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223763于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 30 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 30 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. -
序列相似性
Belongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain. -
翻译后修饰
Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 54472 Human
- Entrez Gene: 54473 Mouse
- Entrez Gene: 361677 Rat
- Omim: 606277 Human
- SwissProt: Q9H0E2 Human
- SwissProt: Q9QZ06 Mouse
- SwissProt: A2RUW1 Rat
- Unigene: 368527 Human
see all -
别名
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
see all
图片
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Western blot - Anti-Tollip antibody (ab223763)All lanes : Anti-Tollip antibody (ab223763) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-2 OS (human brain glioma cell line) cell lysate
Predicted band size: 30 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab223763)Paraffin-embedded human small intestine tissue stained for Tollip using ab223763 at 1/500 dilution in immunohistochemical analysis
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Western blot - Anti-Tollip antibody (ab223763)All lanes : Anti-Tollip antibody (ab223763) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II whole cell lysate
Predicted band size: 30 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Tollip antibody (ab223763)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Tollip (green) using ab223763 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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