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Anti-TNP1抗体(ab73135)

Reviews (2)Q&A (1)References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)

    Key features and details

    • Rabbit polyclonal to TNP1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-TNP1抗体
      参阅全部 TNP1 一抗

    • 描述

      兔多克隆抗体to TNP1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Dog, Pig

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • This antibody gave a positive signal in Human normal testis FFPE section.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab73135于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)

      IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

    文献 (15)

    发表研究结果有使用 ab73135？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab73135 被引用在 15 文献中.

    • Jin Z  et al. The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis. Microbiome 11:212 (2023). PubMed: 37752615
    • Torres-Flores U  et al. Spermiogenesis alterations in the absence of CTCF revealed by single cell RNA sequencing. Front Cell Dev Biol 11:1119514 (2023). PubMed: 37065848
    • Merges GE  et al. Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis. Development 150:N/A (2023). PubMed: 37800308
    • Merges GE  et al. Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice. Development 149:N/A (2022). PubMed: 35608054
    • Wang M  et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
    View all Publications for this product

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNP1 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
    Fixative
    Bouin's solution
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 06 2014

    Western blot abreview for Anti-TNP1 antibody

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Testes)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (NuPage 4-12% Bis-Tris)
    Loading amount
    1 µg
    Specification
    Testes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 08 2013

    Question

    I spoke to a Abcam scientist regarding antibody ab73135 for anti-transition protein #1 yesterday. We are interested in using this antibody for western blotting, though the anitbody apparently has not been validated for such use. He advised us to purchase the antibody, give it a shot, and report back with positive and/or negative results in order to receive credit for a future purchase with Abcam. I ordered the antibody this morning, and was wondering whether I should get the code for the Abtrial program now or wait until I submit the report detailing the antibody's efficacy.

    Read More
    Answer

    I am very pleased to hear you would like to accept our offer and test ab73135 in Western Blot. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for Western Blot. The code is inactive until included in your submitted Abreview so be sure to add it to the Additional Comments section. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/AbTrial

    Read More

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