Anti-TNNT3抗体- C-terminal (ab175058)
Key features and details
- Goat polyclonal to TNNT3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TNNT3抗体- C-terminal
参阅全部 TNNT3 一抗 -
描述
山羊多克隆抗体to TNNT3 - C-terminal -
宿主
Goat -
特异性
ab175058 is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human TNNT3 aa 240-254 (C terminal) (Cysteine residue). The exact sequence is proprietary. NP_006748.1; NP_001036246.1; NP_001036247.1
Sequence:TTLRSRIDQAQKHSK
Database link: P45378 -
阳性对照
- Human, Mouse and Rat skeletal muscle lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab175058 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175058于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 4 - 8 µg/ml.
|
| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 4 - 8 µg/ml. |
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
组织特异性
In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle. -
疾病相关
Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. -
序列相似性
Belongs to the troponin T family. - Information by UniProt
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数据库链接
- Entrez Gene: 7140 Human
- Entrez Gene: 21957 Mouse
- Entrez Gene: 24838 Rat
- Omim: 600692 Human
- SwissProt: P45378 Human
- SwissProt: Q9QZ47 Mouse
- SwissProt: P09739 Rat
- Unigene: 73454 Human
see all -
别名
- AMCD2B antibody
- Beta TnTF antibody
- Beta-TnTF antibody
see all
图片
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Western blot - Anti-TNNT3 antibody - C-terminal (ab175058)All lanes : Anti-TNNT3 antibody - C-terminal (ab175058) at 0.01 µg/ml
Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse skeletal muscle lysate
Lane 3 : Rat skeletal muscle lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 32 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab175058 被引用在 1 文献中.
- Lu J et al. The distal arthrogryposis-linked p.R63C variant promotes the stability and nuclear accumulation of TNNT3. J Clin Lab Anal 35:e24089 (2021). PubMed: 34766372