重组Anti-TNIP2抗体[EPR17434] (ab205925)
![Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-250152-ab205925WBa.jpg "Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17434] to TNIP2
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TNIP2抗体[EPR17434]
参阅全部 TNIP2 一抗 -
描述
兔单克隆抗体[EPR17434] to TNIP2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and HepG2 whole cell lysates; Human fetal liver lysate. ICC/IF: HepG2 and Jurkat cells. IP: HeLa whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17434 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205925于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/90.
|
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| ICC/IF |
1/50.
|
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| IP |
1/30.
|
|
| WB |
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/90. |
|
ICC/IF
1/50. |
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
靶标
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功能
Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. -
组织特异性
Ubiquitously expressed in all tissues examined. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 79155 Human
- Omim: 610669 Human
- SwissProt: Q8NFZ5 Human
- Unigene: 726088 Human
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别名
- A20 binding inhibitor of NF kappaB activation 2 antibody
- A20-binding inhibitor of NF-kappa-B activation 2 antibody
- ABIN 2 antibody
see all
图片
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Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)All lanes : Anti-TNIP2 antibody [EPR17434] (ab205925) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-250151-ab205925WBb.jpg)
Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925)Anti-TNIP2 antibody [EPR17434] (ab205925) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-250150-ab205925IFa.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling TNIP2 with ab205925 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205925 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-250148-ab205925IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (ab205925)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling TNIP2 with ab205925 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205925 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-262002-ab205925FC1.jpg)
Flow Cytometry (Intracellular) - Anti-TNIP2 antibody [EPR17434] (ab205925)Intracellular Flow Cytometry analysis ofJurkat cells labelling TNIP with ab203829 at 1/90 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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![Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-250146-ab205925IP.jpg)
Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (ab205925)TNIP2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab205925 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab205925 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input).
Lane 2: ab205925 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205925 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-TNIP2 antibody [EPR17434] (ab205925)](https://www.abcam.cn/ps/products/205/ab205925/Images/ab205925-8-benefits-of-recombinant-antibodies.png)
Anti-TNIP2 antibody [EPR17434] (ab205925)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab205925 被引用在 2 文献中.
- Roumiguié M et al. PD-L1 expression and pattern of immune cells in pre-treatment specimens are associated with disease-free survival for HR-NMIBC undergoing BCG treatment. World J Urol N/A:N/A (2020). PubMed: 32666225
- Zhang J et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun 10:1858 (2019). PubMed: 31015415