Anti-TNIK抗体(ab95887)
Key features and details
- Rabbit polyclonal to TNIK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TNIK抗体
参阅全部 TNIK 一抗 -
描述
兔多克隆抗体to TNIK -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human TNIK aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_055843.1 -
阳性对照
- HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab95887 was affinity purified using an epitope specific to TNIK immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 155 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 155 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. -
组织特异性
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction. -
细胞定位
Nucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression. - Information by UniProt
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数据库链接
- Entrez Gene: 23043 Human
- Entrez Gene: 665113 Mouse
- Entrez Gene: 294917 Rat
- Omim: 610005 Human
- SwissProt: Q9UKE5 Human
- SwissProt: P83510 Mouse
- Unigene: 34024 Human
- Unigene: 126193 Mouse
see all -
别名
- EC 2.7.11.1 antibody
- KIAA0551 antibody
- Tnik antibody
see all
图片
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Western blot - Anti-TNIK antibody (ab95887)All lanes : Anti-TNIK antibody (ab95887) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 155 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-TNIK antibody (ab95887)Detection of TNIK by Western Blot of Immunprecipitate.
ab95887 at 1µg/ml staining TNIK in HeLa whole cell lysate immunoprecipitated using ab95887 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab95887 尚未被引用在任何文献中。