Anti-TNFRSF14/HVEM抗体(ab47677)
Key features and details
- Rabbit polyclonal to TNFRSF14/HVEM
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-TNFRSF14/HVEM抗体
参阅全部 TNFRSF14/HVEM 一抗 -
描述
兔多克隆抗体to TNFRSF14/HVEM -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Mouse thymus tissue lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47677于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 31 kDa).
|
|
| IHC-P |
Use a concentration of 1 µg/ml.
|
|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 31 kDa). |
|
IHC-P
Use a concentration of 1 µg/ml. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
靶标
-
功能
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. -
组织特异性
Widely expressed, with the highest expression in lung, spleen and thymus. -
序列相似性
Contains 3 TNFR-Cys repeats. -
翻译后修饰
N-glycosylated. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 230979 Mouse
-
别名
- HVEML antibody
- ATAR antibody
- CD270 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody (ab47677)
ab47677 at 1µg/ml staining TNFRSF14/HVEM in mouse thymus by IHC
-
Western blot - Anti-TNFRSF14/HVEM antibody (ab47677)Lane 1 : Anti-TNFRSF14/HVEM antibody (ab47677) at 1 µg/ml
Lane 2 : Anti-TNFRSF14/HVEM antibody (ab47677) at 2 µg/ml
All lanes : Mouse thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody (ab47677)Immunofluorescence of TNFRSF14/HVEM in Mouse Thymus cells using ab47677 at 10 ug/ml.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab47677 被引用在 9 文献中.
- Chen Q et al. The Pyroptosis-Related Risk Genes APOBEC3D, TNFRSF14, and RAC2 Were Used to Evaluate Prognosis and as Tumor Suppressor Genes in Breast Cancer. J Oncol 2022:3625790 (2022). PubMed: 36059808
- Takano G et al. Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma. Mol Ther Oncolytics 20:220-227 (2021). PubMed: 33665360
- Yi Y et al. Clinical significance of herpes virus entry mediator expression in hepatitis B virus-related hepatocellular carcinoma. Oncol Lett 20:19 (2020). PubMed: 32774492
- Tormanen K et al. Restoring Herpesvirus Entry Mediator (HVEM) Immune Function in HVEM-/- Mice Rescues Herpes Simplex Virus 1 Latency and Reactivation Independently of Binding to Glycoprotein D. J Virol 94:N/A (2020). PubMed: 32522859
- Carreras J et al. High TNFRSF14 and low BTLA are associated with poor prognosis in Follicular Lymphoma and in Diffuse Large B-cell Lymphoma transformation. J Clin Exp Hematop 59:1-16 (2019). PubMed: 30918139
- Lan X et al. Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. Onco Targets Ther 10:919-926 (2017). PubMed: 28243127
- Pilling D et al. Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands. BMC Immunol 18:30 (2017). PubMed: 28619036
- Tomita S et al. Genomic and immunohistochemical profiles of enteropathy-associated T-cell lymphoma in Japan. Mod Pathol 28:1286-96 (2015). PubMed: 26226842
- Guo H et al. Herpes virus entry mediator in human corneal epithelial cells modulates the production of inflammatory cytokines in response to HSV type 1 challenge. Ophthalmic Res 54:128-34 (2015). PubMed: 26328884