Anti-TNFRSF10B antibody [RM2078]

• WB

Supplier Data

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab323371 was shown to bind specifically to TNFRSF10B. Target of interest was observed at 40, 48 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in TNFRSF10B knockout cell line (lane 2) (lane 2, knockout cell line ab264922).

The lanes 1-5 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Doxorubicin treatment elevated the expression of DR5 (PMID : 12496481; PMID : 11468181; PMID : 11090076).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-4 : 103 seconds; lane 5-6 : 59 seconds

All lanes:

Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

Lane 1:

Parental HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

TNFRSF10B knockout HeLa whole lysate at 20 µg

Lane 3:

HT-1080 (human fibrosarcoma epithelial cell) whole lysate at 20 µg

Lane 4:

HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole lysate at 20 µg

Lane 6:

HCT 116 treated with 0.5uM doxorubicin for 24 hours whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,48 kDa,36 kDa

true

• WB

Supplier Data

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

NIH/3T3 transfected with siRNA specifically targeting DR5 whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 5:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 59s