Anti-TNFAIP3抗体(ab74037)
Key features and details
- Rabbit polyclonal to TNFAIP3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-TNFAIP3抗体
参阅全部 TNFAIP3 一抗 -
描述
兔多克隆抗体to TNFAIP3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
A synthesized peptide derived from the internal region of human TNFAIP3.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74037于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 90 kDa.
|
|
ICC/IF |
1/500 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 90 kDa. |
ICC/IF
1/500 - 1/1000. |
靶标
-
功能
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. -
序列相似性
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain. -
结构域
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 7128 Human
- Entrez Gene: 21929 Mouse
- Omim: 191163 Human
- SwissProt: P21580 Human
- SwissProt: Q60769 Mouse
- Unigene: 211600 Human
- Unigene: 116683 Mouse
-
别名
- A20 antibody
- AISBL antibody
- MGC104522 antibody
see all
图片
-
All lanes : Anti-TNFAIP3 antibody (ab74037) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
Lane 2 : Extracts from RAW264.7 cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of TNFAIP3 in HepG2 cells using ab74037 at a 1/500 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab74037 被引用在 11 文献中.
- Zhang Y et al. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY) 13:6752-6764 (2021). PubMed: 33621204
- Perga S et al. Overexpression of the ubiquitin-editing enzyme A20 in the brain lesions of Multiple Sclerosis patients: moving from systemic to central nervous system inflammation. Brain Pathol 31:283-296 (2021). PubMed: 33051914
- Hughes TK et al. Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies. Immunity 53:878-894.e7 (2020). PubMed: 33053333
- Jiang W et al. Circ-ATP5H Induces Hepatitis B Virus Replication and Expression by Regulating miR-138-5p/TNFAIP3 Axis. Cancer Manag Res 12:11031-11040 (2020). PubMed: 33173336
- Hu W et al. Der p2-A20 DNA vaccine attenuates allergic inflammation in mice with allergic rhinitis. Mol Med Rep 20:4925-4932 (2019). PubMed: 31638224