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Anti-TNF alpha抗体[V1q(H8)] (ab8097)

Submit a review Q&A (1)References (4)

Key features and details

  • Rat monoclonal [V1q(H8)] to TNF alpha
  • Suitable for: Inhibition Assay, Neutralising, Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgD

概述

  • 产品名称

    Anti-TNF alpha抗体[V1q(H8)]
    参阅全部 TNF alpha 一抗

  • 描述

    大鼠单克隆抗体[V1q(H8)] to TNF alpha

  • 宿主

    Rat

  • 特异性

    Anti-mouse TNF-a reacts with both natural and recombinant TNF-a and shows neutralizing activity.

  • 经测试应用

    适用于: Inhibition Assay, Neutralising, Flow Cytmore details

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Monocytes/macrophages

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab8097于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Inhibition Assay
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration. PubMed: 23300453

The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin.
Flow Cyt
Use at an assay dependent concentration.

Stains the extracellular domain. The CHO cells were fixed in PBA containing 0.2 % formaldehyde before staining.
说明
Inhibition Assay
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration. PubMed: 23300453

The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin.
Flow Cyt
Use at an assay dependent concentration.

Stains the extracellular domain. The CHO cells were fixed in PBA containing 0.2 % formaldehyde before staining.

靶标

  • 功能

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • 疾病相关

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • 序列相似性

    Belongs to the tumor necrosis factor family.

  • 翻译后修饰

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor (TNF superfamily member 2) antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    文献 (4)

    发表研究结果有使用 ab8097？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab8097 被引用在 4 文献中.

    • Helk E  et al. TNFa-mediated liver destruction by kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection. PLoS Pathog 9:e1003096 (2013). Neutralising ; Mouse . PubMed: 23300453
    • Goren I  et al. Systemic anti-TNFalpha treatment restores diabetes-impaired skin repair in ob/ob mice by inactivation of macrophages. J Invest Dermatol 127:2259-67 (2007). PubMed: 17460730
    • Goren I  et al. Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-alpha. Am J Pathol 168:765-77 (2006). PubMed: 16507892
    • Echtenacher B  et al. Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis. J Immunol 145:3762-6 (1990). PubMed: 2246512

    客户评价及客户问答

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    Question

    rat, neutalization assay
    send Abtrial Info

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    Answer

    Thank you for contacting us.

    Here is the information about our Abtrial program.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.


    I hope this information is helpful to you. Please let me know if you like to try one of the antibodies in rat and I can send you the discount code.

    Please do not hesitate to contact us if you need any more advice or information.

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