为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Innate Immunity Cytokines TNF Superfamily

Anti-TNF alpha抗体(ab6671)

  • Datasheet
Reviews (34)Q&A (24)References (846)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-TNF alpha antibody (ab6671)

    Key features and details

    • Rabbit polyclonal to TNF alpha
    • Suitable for: ELISA, IHC-P, ICC/IF, WB, Neutralising
    • Reacts with: Human, Cynomolgus monkey, Recombinant fragment
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-TNF alpha antibody [RM1005] (ab307164)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-TNF alpha抗体
      参阅全部 TNF alpha 一抗
    • 描述

      兔多克隆抗体to TNF alpha
    • 宿主

      Rabbit
    • 特异性

      The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.

      The antibody does not recognize human TNF beta (lymphotoxin).

    • 经测试应用

      适用于: ELISA, IHC-P, ICC/IF, WB, Neutralisingmore details
    • 种属反应性

      与反应: Human, Cynomolgus monkey, Recombinant fragment
      预测可用于: Mouse, Rat, Dog, Pig, Monkey, Non human primates
    • 免疫原

      Recombinant full length protein corresponding to Human TNF alpha.
      Database link: P01375

    • 阳性对照

      • WB: Recombinant TNF-alpha protein.
    • 常规说明

      Endotoxin content by LAL is <10 pg/ml.

      Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.2
      Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
    • Concentration information loading...
    • 纯化说明

      This antibody is primarily directed against mature 17,000 MW human TNF alpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNF alpha in the same formats using similar dilutions.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Signal Transduction
      • Growth Factors/Hormones
      • TNF
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Growth factors
      • TNF
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Metabolism
      • Types of disease
      • Metabolic disorders
      • Neuroscience
      • Development
      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes

    相关产品

    • Affibody® Molecule

      • Anti-TNF alpha Affibody® Molecule (ab31908)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human TNF alpha protein (Active) (ab259410)
      • Recombinant mouse TNF alpha protein (Active) (ab259411)
    • Related Products

      • Rat TNF alpha ELISA Kit (ab100785)
      • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
      • Human TNF alpha ELISA Kit (ab181421)
      • Mouse TNF alpha ELISA Kit (ab208348)
      • Human TNF alpha Matched Antibody Pair Kit (ab213467)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6671于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ELISA
    1/1000 - 1/5000.
    IHC-P (19)
    1/100 - 1/500.
    ICC/IF (1)
    Use at an assay dependent concentration. PubMed: 19458984
    WB (10)
    1/500 - 1/2000. Predicted molecular weight: 25.6 kDa.

    Membrane Blocking is recommended with BSA not Milk for this product.

    Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. 

    Neutralising
    Use at an assay dependent concentration.
    IF
    Use at an assay dependent concentration.
    说明
    ELISA
    1/1000 - 1/5000.
    IHC-P
    1/100 - 1/500.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 19458984
    WB
    1/500 - 1/2000. Predicted molecular weight: 25.6 kDa.

    Membrane Blocking is recommended with BSA not Milk for this product.

    Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. 

    Neutralising
    Use at an assay dependent concentration.
    IF
    Use at an assay dependent concentration.

    靶标

    • 功能

      Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • 疾病相关

      Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • 序列相似性

      Belongs to the tumor necrosis factor family.
    • 翻译后修饰

      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • 细胞定位

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P01375 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7124 Human
      • Entrez Gene: 21926 Mouse
      • Entrez Gene: 24835 Rat
      • Omim: 191160 Human
      • SwissProt: P79337 Cynomolgus monkey
      • SwissProt: P51742 Dog
      • SwissProt: P01375 Human
      • SwissProt: P06804 Mouse
      • SwissProt: P23563 Pig
      • SwissProt: P16599 Rat
      • Unigene: 241570 Human
      • Unigene: 1293 Mouse
      • Unigene: 2275 Rat
      see all
    • 别名

      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • Tnf antibody
      • TNF superfamily member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor (TNF superfamily member 2) antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      see all

    图片

    • Western blot - Anti-TNF alpha antibody (ab6671)
      Western blot - Anti-TNF alpha antibody (ab6671)
      Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF alpha protein

      Secondary
      Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution

      Predicted band size: 25.6 kDa
      Additional bands at: 16 kDa (possible cleavage fragment)



      Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223. 

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (846)

    发表研究结果有使用 ab6671?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6671 被引用在 846 文献中.

    • Lai C  et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
    • Yalcin A  et al. Protective effect of melatonin on learning and memory impairment and hippocampal dysfunction in rats induced by high-fructose corn syrup. Iran J Basic Med Sci 26:69-75 (2023). PubMed: 36594054
    • D'Onofrio N  et al. SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells. Theranostics 13:531-542 (2023). PubMed: 36632236
    • Thangavel H  et al. Susceptibility of Fat Tissue to SARS-CoV-2 Infection in Female hACE2 Mouse Model. Int J Mol Sci 24:N/A (2023). PubMed: 36674830
    • Thangavel H  et al. Diets Differently Regulate Tumorigenesis in Young E0771 Syngeneic Breast Cancer Mouse Model. J Clin Med 12:N/A (2023). PubMed: 36675341
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 58 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: high Target retrival solution
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    FRAU. Ines Kleiber-Schaaf

    Verified customer

    提交于 Nov 04 2020

    Western blot abreview for Anti-TNF alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    4mg/ml bevacizumab for 48hrs
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 09 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (bone)
    Permeabilization
    No
    Specification
    bone
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 28 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Derm)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
    Permeabilization
    Yes - 0.05% triton-x100
    Specification
    Derm
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 28 2019

    Western blot abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 07 2019

    Western blot abreview for Anti-TNF alpha antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Goat Tissue lysate - other (Goat MSCs and Goat Nucleus pulposus cells pellets)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10%)
    Loading amount
    20 µg
    Treatment
    1 ug/mL Lipopolysaccharide induced 3 days
    Specification
    Goat MSCs and Goat Nucleus pulposus cells pellets
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 26°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Chenghao Zhang

    Verified customer

    提交于 Sep 07 2018

    Western blot abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (OV90 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
    Loading amount
    50 µg
    Specification
    OV90 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 18 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (vessel)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: H2O2
    Permeabilization
    No
    Specification
    vessel
    Blocking step
    dako antibody diluent with background reducing components as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 30 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Neuroblastoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 8.5
    Permeabilization
    No
    Specification
    Neuroblastoma
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Allan Wang

    Verified customer

    提交于 Apr 18 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-TNF alpha antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (molar injury)
    Permeabilization
    Yes - Triton X
    Specification
    molar injury
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    提交于 Jan 25 2018

    1-10 of 58 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 4
    • 5
    • ...
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2025 艾博抗(上海)贸易有限公司版权所有