Anti-TNF alpha抗体(ab6671)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: ELISA, IHC-P, ICC/IF, WB, Neutralising
- Reacts with: Human, Cynomolgus monkey, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TNF alpha抗体
参阅全部 TNF alpha 一抗 -
描述
兔多克隆抗体to TNF alpha -
宿主
Rabbit -
特异性
The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.
The antibody does not recognize human TNF beta (lymphotoxin).
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经测试应用
适用于: ELISA, IHC-P, ICC/IF, WB, Neutralisingmore details -
种属反应性
与反应: Human, Cynomolgus monkey, Recombinant fragment
预测可用于: Mouse, Rat, Dog, Pig, Monkey, Non human primates
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免疫原
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375 -
阳性对照
- WB: Recombinant TNF-alpha protein.
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常规说明
Endotoxin content by LAL is <10 pg/ml.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯化说明
This antibody is primarily directed against mature 17,000 MW human TNF alpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNF alpha in the same formats using similar dilutions. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Affibody® Molecule
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/1000 - 1/5000.
|
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| IHC-P | (19) |
1/100 - 1/500.
|
| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 19458984
|
| WB | (10) |
1/500 - 1/2000. Predicted molecular weight: 25.6 kDa.
Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
| Neutralising |
Use at an assay dependent concentration.
|
|
| IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
1/1000 - 1/5000. |
|
IHC-P
1/100 - 1/500. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 19458984 |
|
WB
1/500 - 1/2000. Predicted molecular weight: 25.6 kDa. Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
|
Neutralising
Use at an assay dependent concentration. |
|
IF
Use at an assay dependent concentration. |
靶标
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功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7124 Human
- Entrez Gene: 21926 Mouse
- Entrez Gene: 24835 Rat
- Omim: 191160 Human
- SwissProt: P79337 Cynomolgus monkey
- SwissProt: P51742 Dog
- SwissProt: P01375 Human
- SwissProt: P06804 Mouse
see all -
别名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
图片
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Western blot - Anti-TNF alpha antibody (ab6671)Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF alpha protein
Secondary
Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Predicted band size: 25.6 kDa
Additional bands at: 16 kDa (possible cleavage fragment)Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.
实验方案
数据表及文件
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Datasheet download
文献 (846)
ab6671 被引用在 846 文献中.
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Yalcin A et al. Protective effect of melatonin on learning and memory impairment and hippocampal dysfunction in rats induced by high-fructose corn syrup. Iran J Basic Med Sci 26:69-75 (2023). PubMed: 36594054
- D'Onofrio N et al. SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells. Theranostics 13:531-542 (2023). PubMed: 36632236
- Thangavel H et al. Susceptibility of Fat Tissue to SARS-CoV-2 Infection in Female hACE2 Mouse Model. Int J Mol Sci 24:N/A (2023). PubMed: 36674830
- Thangavel H et al. Diets Differently Regulate Tumorigenesis in Young E0771 Syngeneic Breast Cancer Mouse Model. J Clin Med 12:N/A (2023). PubMed: 36675341
