Anti-TMS1/ASC抗体(ab175449)
Key features and details
- Goat polyclonal to TMS1/ASC
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TMS1/ASC抗体
参阅全部 TMS1/ASC 一抗 -
描述
山羊多克隆抗体to TMS1/ASC -
宿主
Goat -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Mouse TMS1/ASC aa 51-63 (internal sequence) (Cysteine residue). NP_075747.3.
Sequence:DLTDKLVSYYLES
Database link: Q9EPB4 -
阳性对照
- ICC/IF: NIH3T3 cells, KNRK cells and HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab175449 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175449于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
组织特异性
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
序列相似性
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
结构域
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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数据库链接
- Entrez Gene: 282846 Cow
- Entrez Gene: 29108 Human
- Entrez Gene: 66824 Mouse
- Entrez Gene: 282817 Rat
- Omim: 606838 Human
- SwissProt: Q8HXK9 Cow
- SwissProt: Q9ULZ3 Human
- SwissProt: Q9EPB4 Mouse
see all -
别名
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation with ab175449 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab175449 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). -
Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation with ab175449 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab175449 被引用在 37 文献中.
- Gao H et al. Vitamin D3 alleviates inflammation in ulcerative colitis by activating the VDR-NLRP6 signaling pathway. Front Immunol 14:1135930 (2023). PubMed: 36845152
- Liu C et al. Alpha-linolenic acid pretreatment alleviates NETs-induced alveolar macrophage pyroptosis by inhibiting pyrin inflammasome activation in a mouse model of sepsis-induced ALI/ARDS. Front Immunol 14:1146612 (2023). PubMed: 37051243
- Zhao J et al. METTL3-mediated m6 A modification of circPRKAR1B promotes Crohn's colitis by inducing pyroptosis via autophagy inhibition. Clin Transl Med 13:e1405 (2023). PubMed: 37679886
- Zhang Y et al. COG133 Attenuates the Early Brain Injury Induced by Blood-Brain Barrier Disruption in Experimental Subarachnoid Hemorrhage. J Healthc Eng 2022:4404039 (2022). PubMed: 35035834
- Liu C et al. Liquiritin Alleviates Depression-Like Behavior in CUMS Mice by Inhibiting Oxidative Stress and NLRP3 Inflammasome in Hippocampus. Evid Based Complement Alternat Med 2022:7558825 (2022). PubMed: 35069768